普拉克索联合左旋多巴对全脑缺血再灌注损伤大鼠认知能力及线粒体功能的影响

doi:10.3969/j.issn.1006-9771.2023.05.007

Abstract

Abstract:

Objective To investigate the effects of dopamine receptor agonist pramipexole and levodopa on emotion and cognition, and mitochondrial membrane potential of rats after global cerebral ischemia-reperfusion injury.

Methods A total of 80 male Sprague-Dawley rats were divided into sham group (n = 20), model group (n = 20), pramipexole group (n = 20) and combined group (n = 20). The latter three groups were used to prepare the model of global cerebral ischemia-reperfusion injury with Pulsinelli's four-vessel occlusion. The pramipexole group was intraperitoneally injected pramipexole 0.5 mg/kg once a day, while the combined group was injected levodopa 50 mg/kg and pramipexole 0.5 mg/kg, for 14 days. Five rats in each group were tested with open field test three, seven and 14 days after modeling; five were tested with Y-maze test seven and 14 days after modeling; five were detected mitochondrial membrane potential three, seven and 14 days after modeling; and five were observed under Nissl's staining14 days after modeling.

Results Compared with the model group, the number of entries into the central zone (P < 0.05), total distance travelled (P < 0.05) and average velocity (P < 0.05) in the open field test increased in the pramipexole and combined groups seven and 14 days after modeling, duration spent in the central zone increased in the pramipexole and combined groups seven days after modeling (P < 0.05); the rate of spontaneous alternation of Y-maze test increased in the pramipexole and combined groups 14 days after modeling (P < 0.05); mitochondrial membrane potential in hippocampus increased in the pramipexole and combined groups seven and 14 days after modeling (P < 0.05), and it was less in the pramipexole group than in the combined group 14 days after modeling (P < 0.05); and the number of surviving neurons in the hippocampal CA1 increased in the pramipexole and combined groups 14 days after modeling (P < 0.05).

Conclusion Pramipexole may improve emotion and cognition of rats after global cerebral ischemia-reperfusion injury, and it may be helpful for restoring mitochondrial membrane potential as combining with levodopa.

Key words: global cerebral ischemia-reperfusion injury, pramipexole, levodopa, mitochondrial membrane potential, emotion, cognition

CLC Number:

R747.9

KANG Xiaoyu, LIU Lixu, WANG Wenzhu, WANG Yunlei. Effects of pramipexole combined with levodopa on cognitive and mitochondrial function of rats after global cerebral ischemia-reperfusion injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 533-540.

Figures/Tables 8

References 43

[1]	TSAO C W, ADAY A W, ALMARZOOQ Z I, et al. Heart Disease and Stroke Statistics-2023 Update: a report from the American Heart Association[J]. Circulation, 2023, 147(8): e93-e621.
[2]	MYAT A, SONG K J, REA T. Out-of-hospital cardiac arrest: current concepts[J]. Lancet, 2018, 391(10124): 970-979. doi: S0140-6736(18)30472-0 pmid: 29536861
[3]	NABER D, BULLINGER M. Psychiatric sequelae of cardiac arrest[J]. Dialogues Clin Neurosci, 2018, 20(1): 73-77. doi: 10.31887/DCNS.2018.20.1/dnaber
[4]	GEOCADIN R G, WIJDICKS E, ARMSTRONG M J, et al. Practice guideline summary: reducing brain injury following cardiopulmonary resuscitation: report of the Guideline Development, Dissemination, and Implementation Subcommittee of the American Academy of Neurology[J]. Neurology, 2017, 88(22): 2141-2149. doi: 10.1212/WNL.0000000000003966 pmid: 28490655
[5]	WU M Y, YIANG G T, LIAO W T, et al. Current mechanistic concepts in ischemia and reperfusion injury[J]. Cell Physiol Biochem, 2018, 46(4): 1650-1667. doi: 10.1159/000489241
[6]	ANDRABI S S, ALI M, TABASSUM H, et al. Pramipexole prevents ischemic cell death via mitochondrial pathways in ischemic stroke[J]. Dis Model Mech, 2019, 12(8): dmm033860.
[7]	SALMAN M, TABASSUM H, PARVEZ S. Nrf2/HO-1 mediates the neuroprotective effects of pramipexole by attenuating oxidative damage and mitochondrial perturbation after traumatic brain injury in rats[J]. Dis Model Mech, 2020, 13(8): dmm045021.
[8]	WANG W, LIU X, YANG Z, et al. Levodopa improves cognitive function and the deficits of structural synaptic plasticity in hippocampus induced by global cerebral ischemia/reperfusion injury in rats[J]. Front Neurosci, 2020, 14: 586321. doi: 10.3389/fnins.2020.586321
[9]	MÜLLER T, HEFTER H, HUEBER R, et al. Is levodopa toxic?[J]. J Neurol, 2004, 251(Suppl 6): 44-46.
[10]	WANG W, ZHAO L, BAI F, et al. The protective effect of dopamine against OGD/R injury-induced cell death in HT22 mouse hippocampal cells[J]. Environ Toxicol Pharmacol, 2016, 42: 176-182. doi: 10.1016/j.etap.2016.01.020
[11]	SILINDIR M, OZER A Y. The benefits of pramipexole selection in the treatment of Parkinson's disease[J]. Neurol Sci, 2014, 35(10): 1505-1511. doi: 10.1007/s10072-014-1891-5 pmid: 25038745
[12]	MIHAYLOVA A, DONCHEVA N, ZLATANOVA H, et al. Dopaminergic agonist pramipexole improves memory and increases IL-10 production in LPS-challenged rats[J]. Iran J Basic Med Sci, 2021, 24(5): 577-585. doi: 10.22038/ijbms.2021.50439.11488 pmid: 34249258
[13]	SHIN J D, TANG W, JADHAV S P. Dynamics of awake hippocampal-prefrontal replay for spatial learning and memory-guided decision making[J]. Neuron, 2019, 104(6): 1110-1125. doi: S0896-6273(19)30785-8 pmid: 31677957
[14]	EDELMANN E, LESSMANN V. Dopaminergic innervation and modulation of hippocampal networks[J]. Cell Tissue Res, 2018, 373(3): 711-727. doi: 10.1007/s00441-018-2800-7 pmid: 29470647
[15]	KLEIN J, WINTER C, COQUERY N, et al. Lesion of the medial prefrontal cortex and the subthalamic nucleus selectively affect depression-like behavior in rats[J]. Behav Brain Res, 2010, 213(1): 73-81. doi: 10.1016/j.bbr.2010.04.036 pmid: 20434489
[16]	WAHUL A B, JOSHI P C, KUMAR A, et al. Transient global cerebral ischemia differentially affects cortex, striatum and hippocampus in Bilateral Common Carotid Arterial occlusion (BCCAo) mouse model[J]. J Chem Neuroanat, 2018, 92: 1-15. doi: S0891-0618(17)30245-4 pmid: 29702163
[17]	YANG S T, SHI Y, WANG Q, et al. Neuronal representation of working memory in the medial prefrontal cortex of rats[J]. Mol Brain, 2014, 7: 61. doi: 10.1186/s13041-014-0061-2
[18]	DANDUGA R, REDDY D S, SESHADRI S M, et al. Effect of combination therapy with pramipexole and n-acetylcysteine on global cerebral ischemic reperfusion injury in rats[J]. Iran J Basic Med Sci, 2018, 21(6): 569-576. doi: 10.22038/IJBMS.2018.22647.5756 pmid: 29942446
[19]	SEKHON M S, AINSLIE P N, GRIESDALE D E. Clinical pathophysiology of hypoxic ischemic brain injury after cardiac arrest: a "two-hit" model[J]. Crit Care, 2017, 21(1): 90. doi: 10.1186/s13054-017-1670-9
[20]	LAPI D, COLANTUONI A. Remodeling of cerebral microcirculation after ischemia-reperfusion[J]. J Vasc Res, 2015, 52(1): 22-31. doi: 10.1159/000381096 pmid: 25896412
[21]	KALOGERIS T, BAINES C P, KRENZ M, et al. Ischemia/reperfusion[J]. Compr Physiol, 2016, 7(1): 113-170. doi: 10.1002/cphy.c160006 pmid: 28135002
[22]	ARAI N, FURUKAWA N, MIYAMAE T, et al. DOPA cyclohexyl ester, a competitive DOPA antagonist, protects glutamate release and resultant delayed neuron death by transient ischemia in hippocampus CA1 of conscious rats[J]. Neurosci Lett, 2001, 299(3): 213-216. pmid: 11165773
[23]	CASTRO-HERNÁNDEZ J, AFONSO-ORAMAS D, CRUZ-MUROS I, et al. Prolonged treatment with pramipexole promotes physical interaction of striatal dopamine D3 autoreceptors with dopamine transporters to reduce dopamine uptake[J]. Neurobiol Dis, 2015, 74: 325-335. doi: 10.1016/j.nbd.2014.12.007
[24]	DELEU D, NORTHWAY M G, HANSSENS Y. Clinical pharmacokinetic and pharmacodynamic properties of drugs used in the treatment of Parkinson's disease[J]. Clin Pharmacokinet, 2002, 41(4): 261-309. pmid: 11978145
[25]	MA J, WANG Z, LIU C, et al. Pramipexole-induced hypothermia reduces early brain injury via PI3K/AKT/GSK3β pathway in subarachnoid hemorrhage rats[J]. Sci Rep, 2016, 6: 23817. doi: 10.1038/srep23817 pmid: 27026509
[26]	MISSALE C, NASH S R, ROBINSON S W, et al. Dopamine receptors: from structure to function[J]. Physiol Rev, 1998, 78(1): 189-225. doi: 10.1152/physrev.1998.78.1.189 pmid: 9457173
[27]	KLINKER F, HASAN K, PAULUS W, et al. Pharmacological blockade and genetic absence of the dopamine D2 receptor specifically modulate voluntary locomotor activity in mice[J]. Behav Brain Res, 2013, 242: 117-124. doi: 10.1016/j.bbr.2012.12.038 pmid: 23291158
[28]	LUIS-RAVELO D, FUMAGALLO-READING F, CASTRO-HERNANDEZ J, et al. Prolonged dopamine D(3) receptor stimulation promotes dopamine transporter ubiquitination and degradation through a PKC-dependent mechanism[J]. Pharmacol Res, 2021, 165: 105434. doi: 10.1016/j.phrs.2021.105434
[29]	CHERNOLOZ O, EL M M, BLIER P. Sustained administration of pramipexole modifies the spontaneous firing of dopamine, norepinephrine, and serotonin neurons in the rat brain[J]. Neuropsychopharmacology, 2009, 34(3): 651-661. doi: 10.1038/npp.2008.114 pmid: 18688211
[30]	CHERNOLOZ O, EL M M, BLIER P. Long-term administration of the dopamine D3/2 receptor agonist pramipexole increases dopamine and serotonin neurotransmission in the male rat forebrain[J]. J Psychiatry Neurosci, 2012, 37(2): 113-121. doi: 10.1503/jpn.110038 pmid: 22023785
[31]	LIEBERKNECHT V, CUNHA M P, JUNQUEIRA S C, et al. Antidepressant-like effect of pramipexole in an inflammatory model of depression[J]. Behav Brain Res, 2017, 320: 365-373. doi: S0166-4328(16)31010-5 pmid: 27825895
[32]	MIHAYLOVA A S, KOSTADINOV I D, DONCHEVA N D, et al. Effects of pramipexole on learning and memory processes in naive and haloperidol-challenged rats in active avoidance test[J]. Folia Med (Plovdiv), 2019, 61(2): 258-265.
[33]	CHOI E B, KIM J Y, JANG S H. Motor recovery of hemiparetic leg by improvement of limb-kinetic apraxia in a chronic patient with traumatic brain injury[J]. Medicine, 2020, 99(19): e20144. doi: 10.1097/MD.0000000000020144
[34]	LAN Y L, LI S, LOU J C, et al. The potential roles of dopamine in traumatic brain injury: a preclinical and clinical update[J]. Am J Transl Res, 2019, 11(5): 2616-2631.
[35]	SAYEED I, PARVEZ S, WINKLER-STUCK K, et al. Patch clamp reveals powerful blockade of the mitochondrial permeability transition pore by the D2-receptor agonist pramipexole[J]. FASEB J, 2006, 20(3): 556-558. doi: 10.1096/fj.05-4748fje pmid: 16407457
[36]	CASSARINO D S, FALL C P, SMITH T S, et al. Pramipexole reduces reactive oxygen species production in vivo and in vitro and inhibits the mitochondrial permeability transition produced by the Parkinsonian neurotoxin methylpyridinium ion[J]. J Neurochem, 1998, 71(1): 295-301. pmid: 9648878
[37]	GU M, IRAVANI M M, COOPER J M, et al. Pramipexole protects against apoptotic cell death by non-dopaminergic mechanisms[J]. J Neurochem, 2004, 91(5): 1075-1081. pmid: 15569251
[38]	VIARO R, LONGO F, VINCENZI F, et al. l-DOPA promotes striatal dopamine release through D1 receptors and reversal of dopamine transporter[J]. Brain Res, 2021, 1768: 147583. doi: 10.1016/j.brainres.2021.147583
[39]	ZOU L, JANKOVIC J, ROWE D B, et al. Neuroprotection by pramipexole against dopamine- and levodopa-induced cytotoxicity[J]. Life Sci, 1999, 64(15): 1275-1285. pmid: 10227583
[40]	YANG P, PERLMUTTER J S, BENZINGER T, et al. Dopamine D3 receptor: a neglected participant in Parkinson disease pathogenesis and treatment?[J]. Ageing Res Rev, 2020, 57: 100994. doi: 10.1016/j.arr.2019.100994
[41]	MISHRA A, SINGH S, TIWARI V, et al. Dopamine D1 receptor agonism induces dynamin related protein-1 inhibition to improve mitochondrial biogenesis and dopaminergic neurogenesis in rat model of Parkinson's disease[J]. Behav Brain Res, 2020, 378: 112304. doi: 10.1016/j.bbr.2019.112304 pmid: 31626851
[42]	MISHRA A, SINGH S, TIWARI V, et al. Dopamine receptor activation mitigates mitochondrial dysfunction and oxidative stress to enhance dopaminergic neurogenesis in 6-OHDA lesioned rats: a role of Wnt signalling[J]. Neurochem Int, 2019, 129: 104463. doi: 10.1016/j.neuint.2019.104463
[43]	WILSON S M, WURST M G, WHATLEY M F, et al. Classics in chemical neuroscience: pramipexole[J]. ACS Chem Neurosci, 2020, 11(17): 2506-2512. doi: 10.1021/acschemneuro.0c00332 pmid: 32786316

组别	n	3 d	7 d	14 d
假手术组	5	15.80±4.21	16.60±9.21	11.60±7.99
模型组	5	1.60±0.89^a	2.00±1.00^a	3.00±1.73^a
普拉克索组	5	5.60±2.61^a,b	6.20±2.17^a,b	9.20±4.44^b
联合组	5	4.80±2.28^a,b	6.00±4.64^b	9.80±6.98^b
χ²值		15.354	13.745	8.191
P值		0.002	0.003	0.042

组别	n	3 d	7 d	14 d
假手术组	5	36.13±17.43	24.08±8.24	22.07±4.36
模型组	5	7.54±4.52	2.99±1.70^a	4.11±1.49^a
普拉克索组	5	11.30±6.32	16.43±9.06^b	20.06±16.47
联合组	5	19.13±8.94	9.58±3.69^a	19.42±13.80
F值		7.251	9.854	2.839
P值		0.003	0.001	0.071

组别	n	3 d	7 d	14 d
假手术组	5	7513.9±691.1	5925.5±1057.1	5440.4±1116.3
模型组	5	2517.3±249.7^a	2311.7±407.6^a	2744.6±682.9^a
普拉克索组	5	3907.7±649.2^a,b	4231.6±539.3^a,b	5249.9±1322.5^b
联合组	5	3185.0±483.0^a	4259.0±823.1^a,b	4821.7±498.6^b
F值		83.164	19.379	8.293
P值		< 0.001	< 0.001	0.001

组别	n	3 d	7 d	14 d
假手术组	5	25.16±2.30	19.82±3.54	18.20±3.74
模型组	5	8.42±0.83^a	7.76±1.36^a	9.20±2.27^a
普拉克索组	5	13.08±2.16^a,b	14.16±1.79^a,b	17.60±4.43^b
联合组	5	10.68±1.61^a	14.24±2.76^a,b	16.16±1.67^b
F值		83.988	19.314	8.274
P值		< 0.001	< 0.001	0.002

组别	n	7 d	14 d
假手术组	5	80.66±5.10	80.83±4.95
模型组	5	44.25±11.69^a	52.83±5.51^a
普拉克索组	5	82.72±8.22^b	73.45±10.79^b
联合组	5	72.25±3.71^b	74.73±6.99^b
F值		25.771	13.492
P值		< 0.001	< 0.001

Effects of pramipexole combined with levodopa on cognitive and mitochondrial function of rats after global cerebral ischemia-reperfusion injury

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 43

Related Articles 15

Metrics

Comments

Recommended 0

组别	n	3 d	7 d	14 d
假手术组	5	70.38±7.97	73.04±6.96	97.36±7.49
模型组	5	66.71±0.91	47.56±3.61^a	61.66±3.69^a
普拉克索组	5	69.67±5.65	72.66±1.47^b	71.45±1.19^a,b
联合组	5	70.71±18.87	74.33±6.39^b	81.15±4.12^a,b,c
F值		0.147	31.935	52.592
P值		0.930	< 0.001	< 0.001

组别	n	存活神经元数
假手术组	5	114.40±2.70
模型组	5	74.20±3.56^a
普拉克索组	5	107.20±3.11^a,b
联合组	5	103.80±3.56^a,b
F值		147.679
P值		< 0.001

[1]	WANG Haoyi, SHI Yawei, LU Jun, XU Guangxu. Impact of subjective vertical perception impairment on function in stroke patients: a retrospective study [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2024, 30(1): 68-73.
[2]	JIANG Changhao, HUANG Chen, GAO Xiaoyan, DAI Yuanfu, ZHAO Guoming. Effect of neurofeedback training on cognitive function in the elderly: a systematic review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(8): 903-909.
[3]	LIU Yang, ZHANG Peng, HUANG Ying, CHEN Han, XU Chen, LI Min. Path analysis of mediating effect of perceived stress affecting impact of event in rehabilitation patients with traumatic injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(8): 954-960.
[4]	JIANG Changhao, GAO Xiaoyan. Effect of acute physical activity on cognitive function in children: a systematic review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(6): 667-672.
[5]	ZHU Xiaomin, LIU Huilin, LIU Yuanmin, YAN Zhiyu, DU Xuejing, WANG Ya'nan, ZHANG Tong. Relationship among spontaneous turning direction, balance and fall risk in stroke patients during walking [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 510-515.
[6]	WANG Junsheng, MENG Jiao, GUO Zizhao, JIANG Changhao. Effects of mind-body exercise on sleep quality: a systematic review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(2): 205-213.
[7]	ZHU Xu,LIU Jing,DONG Zeping,QIU Dawei. Gesture action intent recognition based on surface electromyography: a systematic review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(9): 1032-1038.
[8]	ZHANG Xiaoyu,YANG Fan,WEN Jianzhong,YU Weiyong. Application of resting-state functional magnetic resonance imaging in acute mild traumatic brain injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(9): 1084-1088.
[9]	XUAN Wenru,SHEN Yuqing,ZHOU Miao,FENG Shiwen. Bilingual training for cognition of older adults: a systematic review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 578-584.
[10]	ZHONG Xiaoke,ZHANG Ji,WANG Zhipeng,JIANG Changhao. Effect of physical activity on neurocognitive function of overweight children: a systematic review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(4): 421-428.
[11]	LIU Fengbo,ZHANG Zhongqiu,LI Anqiao,ZHOU Sheng,ZHANG Meng. Effects of mindfulness training on emotion and acceptance for people with disabilities: a systematic review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(1): 105-110.
[12]	LIN Ai-jin,WANG Jie-qiong,AO Li-juan,CHEN Mo-xian. Long-term Behavioral Disorder after Hypoxic Ischemic Brain Damage in Newborn Mice [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(8): 908-912.
[13]	WANG Jin-fang,SHI Qing-li,CHEN Hong-yan,WANG Shi-nan,YAO Jing-fan,FENG Li,ZHANG Yu-mei. Relationship between Small-world Network and Cognitive Impairment for Patients with White Matter Lesions Based On Diffusion Tensor Imaging [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(7): 780-784.
[14]	Ting-ting CHEN,Ming-chao XU,Rou WEN. Effects of Dance Activity on Negative Emotion in Older Adults: A Systematic Review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 668-676.
[15]	Xin LIU,Zhi-ke YIN,Tao FENG,Yong-mei DENG,Yue-ying ZHAO,Ke DONG,Chun-xue WANG,Hui-zi MA. Risk Factors of Apathy in Parkinson's Disease [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 719-723.