不同针刺方案对卒中后抑郁大鼠神经递质及相关炎性因子的影响

doi:10.3969/j.issn.1006-9771.2023.01.005

Abstract

Abstract:

Objective To investigate the effects of different acupuncture schemes on behaviors, neurotransmitters and inflammation-related factors in post-stroke depressed (PSD) rats.

Methods A total of 72 healthy male Sprague-Dawley rats were randomly divided into normal group, model group, drug control group, scalp acupuncture group, abdominal acupuncture group and combined acupuncture group, 12 rats in each group. The PSD model was prepared using a combination of middle cerebral artery occlusion and chronic unpredictable mild stimulation (CUMS) for the model and each intervention groups. The drug control group was administered fluoxetine, the scalp acupuncture group accepted acupuncture at Baihui (DU 20) and Yintang (EX-HN3), while the abdominal acupuncture group at Zhongwan (RN 12) and Guanyuan (RN 4), the combined acupuncture group at all the four acupoints, for 21 days. They were assessed with Longa neurological function score, body mass, open-field test and sugar-water preference test on the 0 (before modeling), the seventh (before CUMS), the 14th (before treatment) and the 35th day (after treatment). The levels of 5-hydroxytryptamine (5-HT), dopamine (DA), norepinephrine (NE), interferon-γ (INF-γ), tumor necrosis factor-α (TNF-α) and macrophage migration inhibitory factor (MIF) in the serum were determined with ELISA on the 35th day.

Results The neurological function score was lower in the drug control group and the combined acupuncture group than in the model group (P < 0.01) on the 35th day; while the body mass was higher in the drug control group, the abdominal acupuncture group and the combined acupuncture group than in the model group (P < 0.01); and the number of horizontal span frames, the number of uprightness and the sugar water consumption were higher in the drug control group, the scalp acupuncture group, the abdominal acupuncture group and the combined acupuncture group than in the model group (P < 0.05). There was no significant difference among the four intervention groups (P > 0.05). The levels of 5-HT, DA and NE were higher in the four intervention groups than in the model group (P < 0.01), and the levels of IFN-γ, TNF-α and MIF were lower (P < 0.01). 5-HT level was higher in the combined acupuncture group than in the scalp acupuncture group and the abdominal acupuncture group, and the levels of IFN-γ, TNF-α and MIF were lower (P< 0.01); the levels of DA and NE was higher in the combined acupuncture group than in the abdominal acupuncture group (P < 0.01).

Conclusion The combination of scalp acupuncture and abdominal acupuncture can improve behavior, neurotransmitters and inflammatory factors of PSD rats, like fluoxetine. Scalp or abdominal acupuncture alone is still effecive, and can be used secondarily.

Key words: post-stroke depression, acupuncture, neurotransmitter, inflammatory factors, rats

CLC Number:

R743.3

QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.

Figures/Tables 3

References 38

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组别	n	5-HT	DA	NE
正常组	6	35.03±1.60	22.37±2.77	32.60±1.82
模型组	6	9.47±0.43^a	7.18±0.44^a	7.57±0.26^a
药物对照组	6	29.22±2.29^a,b	15.67±2.80^a,b	20.62±1.33^a,b
头针组	6	11.68±0.39^a,b,c	14.23±0.86^a,b	11.89±0.94^a,b,c
腹针组	6	12.23±0.51^a,b,c	11.41±0.97^a,b,d	9.71±0.35^a,b,c,d
联合针刺组	6	25.11±0.88^a,b,d,e	14.93±0.77^a,b,e	12.38±0.37^a,b,c,e
F值		452.836	50.728	500.749
P值		< 0.001	< 0.001	< 0.001

组别	n	IFN-γ	TNF-α	MIF
正常组	6	908.653±160.739	893.502±55.215	114.468±3.029
模型组	6	1695.264±44.758^a	4045.549±250.484^a	258.007±5.574^a
药物对照组	6	1020.900±108.573^b	2015.923±196.053^a,b	175.023±7.115^a,b
头针组	6	1064.978±19.515^b	2399.542±143.557^a,b,c	194.047±4.755^a,b,c
腹针组	6	1197.505±16.278^b,d	2641.162±133.778^a,b,c	205.421±4.337^a,b,c,d
联合针刺组	6	1011.452±7.722^b,d,e	2035.327±116.839^a,b,d,e	179.145±6.569^a,b,d,e
F值		71.567	243.707	447.072
P值		< 0.001	< 0.001	< 0.001

Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression

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