有氧运动对睡眠剥夺大鼠学习记忆及海马神经元突触可塑性的效果

doi:10.3969/j.issn.1006-9771.2022.11.006

Abstract

Abstract:

Objective To investigate the effects of aerobic exercise on sleep-deprived rats positioning cruise and space exploration activities, synaptic mechanism, involving hippocampal neuron morphology, dendritic spine density, synaptic related proteins and cyclic adenosine phosphate reactive element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway.

Methods A total of 48 Sprague-Dawley rats were randomly divided into control group (n = 12), model group (n = 12), model-exercise group (n = 12) and exercise group (n = 12). The model-exercise group and the exercise group accepted aerobic exercise with treadmill for four weeks. After exercise intervention, the model group and the model-exercise group were continuously deprived sleep for 72 hours with a multi-platform water environment. They were assessed with Morris Water Maze of positioning cruise and space exploration. The morphology and density of dendritic spines in hippocampal neurons were observed with HE staining and Golgi staining. The expression of hippocampal proteins of postsynaptic density protein 95 (PSD95), synapsin (SYN), growth-associated protein 43 (GAP43), Rac1, BDNF, p-CREB and CREB were measured with Western blotting.

Results Compared with the control group, the latency and the total swimming distance increased in the model group (P< 0.05); the number of crossing the platform, the target quadrant residence time and distance decreased (P< 0.05); the neurons in the hippocampal CA1 region were disordered, the total density of dendritic spines per unit length and the density of mature, immature and filamentous pseudopodia dendritic spines decreased (P< 0.05); and the expression of PSD95, SYN, GAP43, Rac1, BDNF, p-CREB and CREB decreased (P< 0.05). Compared with the model group, the latency and total swimming distance of the model-exercise group decreased (P< 0.05); the target quadrant residence time and distance increased (P< 0.05); the number of neurons in the hippocampal CA1 region increased and arranged neatly; the total density of dendritic spines per unit length and the density of mature, immature and filamentous pseudopod dendritic spines increased (P< 0.05); and the expression of PSD95, SYN, GAP43, Rac1, BDNF, p-CREB and CREB increased (P< 0.05).

Conclusion Aerobic exercise can alleviate the learning and memory impairment of sleep-deprived rats, which may relate to the regulation of CREB/BDNF signaling pathway in hippocampus for improvement of synaptic plasticity.

Key words: sleep deprivation, aerobic exercise, synaptic plasticity, learning and memory, rats

CLC Number:

LI Tong,FANG Zhipeng,SHAO Yuping,WANG Ping. Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1270-1277.

Figures/Tables 8

References 40

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组别	n	潜伏期/s	游泳总距离/cm	穿越平台次数/次	目标象限距离/cm	目标象限时间/s
对照组	12	26.39±9.33	376.32±42.78	4.41±0.70	419.97±42.18	29.69±3.09
模型组	12	51.18±12.46^a	586.02±51.79^a	1.82±0.59^a	323.84±44.47^a	21.38±1.88^a
模型运动组	12	32.20±7.14^b	493.53±42.68^b	3.49±0.82^b	365.73±19.63^b	26.09±2.06^b
运动组	12	22.16±9.66	361.25±33.21	4.86±0.81	443.36±39.04	32.48±2.49
F值		17.303	56.723	36.492	22.514	40.907
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	总密度	成熟型	不成熟型	丝状伪足型
对照组	6	15.63±1.77	7.82±0.88	6.25±0.71	1.71±0.13
模型组	6	8.04±0.92^a	2.49±0.27^a	4.02±0.46^a	1.36±0.15^a
模型运动组	6	12.53±1.10^b	5.03±0.44^b	4.64±0.41^b	1.56±0.18^b
运动组	6	17.14±1.34	8.57±0.67	6.86±0.54	1.88±0.13
F值		72.976	170.371	49.148	16.417
P值		< 0.001	< 0.001	< 0.001	< 0.001

组别	n	PSD95	SYN1	GAP43	Rac1
对照组	6	61.57±2.99	57.25±3.76	54.88±3.77	51.02±1.97
模型组	6	38.11±2.29^a	34.61±2.06^a	31.60±1.57^a	41.05±2.32^a
模型运动组	6	56.01±3.45^b	42.82±2.87^b	44.20±2.74^b	44.61±2.92^b
运动组	6	72.06±3.58	61.97±2.56	62.13±2.87	53.08±2.95
F值		62.189	58.118	65.320	42.865
P值		< 0.001	< 0.001	< 0.001	< 0.001

组别	n	BDNF	p-CREB	CREB
对照组	6	90.59±1.54	35.49±4.36	62.13±2.69
模型组	6	79.97±1.39^a	7.47±1.03^a	42.55±3.19^a
模型运动组	6	84.46±1.93^b	22.44±3.16^b	53.54±3.29^b
运动组	6	94.47±2.02	41.18±3.02	63.86±2.63
F值		41.037	68.771	26.700
P值		< 0.001	< 0.001	< 0.001

Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats

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