迷走神经刺激对脑缺血再灌注大鼠磷酸腺苷活化蛋白激酶-沉默信息调节因子2相关酶1自噬通路的影响

doi:10.3969/j.issn.1006-9771.2020.07.007

Abstract

Abstract:

Objective To investigate the effect of vagus nerve stimulation (VNS) on the autophagy in penumbra of rats after middle cerebral artery occlusion (MCAO). Methods A total of 56 Sprague-Dawley rats were divided into control group (n = 14), sham group (n = 14), model group (n = 14) and VNS group (n = 14). The model group and VNS group accepted MCAO for one hour, then reperfusion, and VNS group accepted VNS in the left as MCAO for 0.5 hour. Their infarct volume was detected with TTC staining, neurological impairment was assessed with Longa's score, and the expression of Beclin-1, microtubule-associated protein 1 light chain 3 (LC3)-II/I, phosphorylated adenosine monophosphate activated protein kinase (P-AMPK) and silent mating type information regulation 2 homolog 1 (Sirt1) were detected with Western blotting 24 hours after reperfusion.Results Compared with the sham group, the infarct volume and Longa's score increased in the model group, while the expression of Beclin-1, LC3-II/I, P-AMPK and Sirt1 decreased significantly (P < 0.001). Compared with the model group, the infarct volume and Longa's score reduced ( P < 0.05) in VNS group, while the expression of Beclin-1, LC3-II/I, P-AMPK and Sirt1 increased significantly ( P < 0.001). Conclusion VNS may alleviate cerebral ischemic injury in rats through AMPK-Sirt1 pathway.

Key words: ischemic stroke, vagus nerve electrostimulation, autophagy, adenosine monophosphate activated protein kinase, pathway

CLC Number:

R743.3

WEI Hai-ping,GUO Jia,WANG Huan,GE Zhao-ming. Effects of Vagus Nerve Stimulation on Adenosine Monophosphate Activated Protein Kinase-Silent Mating Type Information Regulation 2 Homolog 1 Pathway in Ischemia-reperfusion Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(7): 775-779.

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References 25

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组别	n	梗死体积
正常组	4	0
假手术组	4	0
模型组	4	41.360±3.309
VNS组	4	21.229±3.815^a
F值		47.674
P值		< 0.001

组别	n	Longa评分
正常组	5	0.600±0.490
假手术组	5	0.800±0.400
模型组	5	7.200±2.040^a
VNS组	5	4.600±0.800^a,b
F值		25.065
P值		< 0.001

组别	n	Beclin-1	LC3-II/LC3-I	P-AMPK	Sirt1
正常组	5	100.00±0.00	100.00±0.00	100.00±0.00	100.00±0.00
假手术组	5	100.68±4.46	101.66±9.90	96.40±3.91	99.23±9.27
模型组	5	34.56±5.95^a	27.69±5.24^a	25.64±3.71^a	29.22±4.15^a
VNS组	5	76.10±7.47^a,b	66.29±8.53^a,b	65.80±2.65^a,b	66.57±8.67^a,b
F值		138.623	82.833	481.782	82.544
P值		< 0.001	< 0.001	< 0.001	< 0.001

Effects of Vagus Nerve Stimulation on Adenosine Monophosphate Activated Protein Kinase-Silent Mating Type Information Regulation 2 Homolog 1 Pathway in Ischemia-reperfusion Rats

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