《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (03): 182-183.

• 基础研究 • 上一篇    下一篇

借助人工神经修复大鼠坐骨神经缺损的实验研究

杨朝阳1; 蔡青2; 鲁强2; 姬曼2; 王元身2; 高尔静2; 刘玉军2; 李晓光2   

  1. 1.宁夏医学院解剖教研室 宁夏银川市 750004;2.首都医科大学北京神经科学研究所 北京市 100054
  • 收稿日期:2002-12-27 出版日期:2003-03-25 发布日期:2003-03-25

Repair sciatic nerve gap of the rats with novel artifical nerve guide

YANG Zhao-yang,CAI Qing,LU Qiang,et al   

  1. Beijing Center for Neural Regeneration and Repairing,Capital University of Medical Science.Beijing 100054,China
  • Received:2002-12-27 Published:2003-03-25 Online:2003-03-25

PDF (PC)

被引次数

7

摘要： 目的研究人工神经-壳聚糖复合胶原导管修复大鼠坐骨神经15mm缺损的可行性。方法借助人工神经修复10只大鼠坐骨神经缺损15mm,神经缺损7只为对照组,术后2个月、4个月行免疫组化、Osmium染色、Bodian染色、运动终板的特异染色、WGA-HRP神经示踪及大体观察。结果术后2个月再生神经修复了坐骨神经的缺损,实验动物未出现排斥反应及明显的炎症反应。结论人工神经导管对缺损的坐骨神经修复具有良好的桥梁作用和促神经生长的作用。

关键词: 人工神经, 壳聚糖复合胶原导管, 神经再生, 大鼠, 坐骨神经

Abstract: ObjectiveTo explore the possibility of repairing sciatic nerve gap of rats with artifical nerve graft.MethodsA novel artifical nerve guide was developed and used to suture the 15 milimeter long right sciatic nerve gap of 10 rats, other 7 rats were the control with the right sciatic nerve gap alone.2 and 4 monthes after operation, immunohistochemistry, Osmium staining, Bodian staining,motor end plate staining,WGA-HRP stain tracing have been done to observe the effect of repairing.Results2 months after operations, the sciatic nerve gap were repaired by the regeneration nerve.There was not evident inflammation in the defects.ConclusionsThe artifical nerve graft can induce the nerve to regenerate.

Key words: the artifical nerve, nerve regeneration, sciatic nerve, rats