《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (03): 176-178.

• 基础研究 • 上一篇    下一篇

应用壳聚糖导管生物活性载体系统修复大鼠脊髓损伤

李晓光; 杨朝阳; 张皑峰; 鲁强; 蔡青; 王元身; 刘玉军; 徐群渊; 刘岩; 陈惟昌   

  1. 首都医科大学北京神经科学研究所 北京市 100054
  • 收稿日期:2002-12-27 出版日期:2003-03-25 发布日期:2003-03-25

Repair of spinal cord lesion of rats by using of chitosan tube combined with the bio-active carrier system

LI Xiao-guang,YANG Zhao-yang,ZHANG Ai-feng,et al   

  1. Beijing Centerfor Neural Regeneration and Repairing,Capital University of Medical Sciences.Beijing 100054,China
  • Received:2002-12-27 Published:2003-03-25 Online:2003-03-25

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摘要： 目的研究壳聚糖导管-生物活性载体系统诱导神经再生的情况。方法 50只成年雌性Wister大鼠脊髓T7—9节段行脊髓右侧半离断,分别植入含有(40只)或不含有(10只 )生物活性载体的壳聚糖导管作桥梁,缝合硬脊膜,恢复脑脊液循环。结果6—12个月后,在含有生物活性载体的导管中脊髓缺损部位神经再生完成,经过WGA-HRP进行顺行神经束路追踪,发现再生轴索已到达缺损部位的远端,并越过导管远端与宿主的界面进入损伤平面以下脊髓组织,超微结构观察到典型的神经纤维、髓鞘结构和突触。在不含生物活性载体的导管中,再生的轴突很少,且没有轴突穿过导管中点。结论内含生物活性载体的生物材料导管可诱导大鼠脊髓神经轴突的再生。

关键词: 脊髓损伤, 壳聚糖导管, 生物活性载体, 神经再生, 大鼠

Abstract: ObjectiveTo study the effect the chitosan tube combined with the bio active carrier system on inducing nerve axon regeneration of rats with spinal cord injury.Methods50 female Wister rats were characterized by the right spinal cord hemisections at the seventh and eighth thoracic segment to make the model of spinal cord hemisection. The chitosan tube serving as a regenerative loculus was implanted in the defect location of the experimental models.In the experimental group,the bio active carrier system was injected into the chitosan tube,while in control group injected nothing.The drua was sutured to restore cerebrospinal fluid circulation.Results6 months and 12 months after operation,the regenerative nerve axon had passed the defect area of spinal cord in the experiment group. According to WGA-HRP anterograde axonal tracing study, some TMB-positive axons were observed in the distal graft host interface,and came into the host environment. TEM-ultrastructure indicate some neonate synapse, myelinated nerve fibers.In the control group,few regenerative axon could be seen, there was no regenerative axon pass the middle of the tube.ConclusionsThe chitosan tube in which the bio active carrier system was injected can induce the spinal cord nerve axon regeneration.

Key words: spinal cord lesion, chitosan tube, nerve regeneration, bio active carrier, rats