《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (11): 665-666.

• 基础研究 • 上一篇    下一篇

低功率毫米波对荷瘤小鼠红细胞免疫黏附活性的影响及作用分析

李雪平1; 陈光1; 陈景藻2; 杨红3; 郭峰3   

  1. 1.南京军区南京总医院康复医学科 江苏南京市 210001;2.第四军医大学 陕西西安市 710032;3.第二军医大学 上海市 200433
  • 收稿日期:2002-05-31 出版日期:2002-11-25 发布日期:2002-11-25

Effect of low-intensity millimeter wave irradiation on the immune adhesion function of erythrocytes in mice with tumor

LI Xue-ping,CHEN Guang,CHEN Jing-zhao,et al   

  1. Nanjing Military General Hospital, Nanjing 210002,Jiangshu,China
  • Received:2002-05-31 Published:2002-11-25 Online:2002-11-25

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摘要： 目的研究毫米波(MMW)对荷瘤小鼠红细胞免疫粘附功能的影响。方法健康雄性昆明小鼠60只,体重(16±2)g ,随机分为两组,每组30只。两组小鼠腹腔接种浓度为1×106/ml小鼠艾氏腹水癌细胞0.2ml,接种后当天开始,辐射组用频率36GHz,功率密度0.73—1.46mW/cm2毫米波辐射小鼠背部30min,每日1次,连续8天。对照组在同等条件下进行假辐射。于辐射后0d、3d、6d分别取辐射组及对照组小鼠各10只,抽取静脉血,分别测定RBC-C3b受体花环率(RCR)、tumour RBC花环率(TRR)、RBC-SOD活性及RBC-LPO含量。结果在辐射后3天时,辐射组的RCR、TRR显著高于对照组(P<0.01),RBC-LPO含量下降明显(P<0.05),RBC-SOD活性无明显增加(P>0.05);在辐射后0天和6天时,辐射组各测定指标与对照组相比无显著差异(P>0.05)。结论低功率毫米波辐射可促进荷瘤小鼠红细胞免疫粘附功能,减轻红细胞的脂质过氧化损伤,其免疫学效应与时间有关。

关键词: 毫米波辐射, 免疫黏附, 肿瘤, 红细胞, 小鼠

Abstract: ObjectiveTo study the effect of low-intensity millimeter wave(MMW)irradiation on the immune adhesion function of erythrocytes in mice with tumor.Methods60 healthy male mice were randomized into two groups:irradiation group(n=30) and control group(n=30).All mice were injected into abdominal cavity with A's abdominal juice cancer-cell(1×106/ml,0.2ml).MMW(36GHz,0.73-1.46mw/cm2)was used to irradiate the back of mice in irradiation group,once a day for 8 days.Control group received false irradiation using the same method.At 0d,3d,6d after irradiation, the erythrocytes were extracted from vein blood in the mice,and the RBC-C3b receptor rosettes rate(RCR),tumour-RBC rosette rate(TRR),RBC-SOD and RBC-LPO were determined.ResultsAt 3d after radiation,the RBC-C3b and TRR of irradiation group were higher than that of control group (P<0.01) and RBC-LPO was lower (P<0.05),while there was no significant difference on activity of RBC-SOD(P>0.05). there were no significant difference on all index between two groups at 0d and 6d(P>0.05).ConclusionsLow-intensity millimeter wave irradiation can improve the immune adhesion function of erythrocytes in mice with tumor.

Key words: millimeter-wave(MMW), tumour, RBC, Immunity adhesion, mice