《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (11): 661-663.

• 基础研究 • 上一篇    下一篇

次声作用后大鼠延髓星形胶质细胞和神经元反应的关系

袁华; 龙华; 李玲; 牟翔; 邱建勇; 刘静; 陈景藻   

  1. 第四军医大学附属西京医院理疗与康复科 陕西西安市 710032
  • 收稿日期:2002-10-30 出版日期:2002-11-25 发布日期:2002-11-25

Relationship between rats' medullary astrocytes and neurons after exposed to infrasound

YUAN Hua, LONG Hua, LI Ling,et al   

  1. Department of Physiotherapy and Rehabilitation, XijingHospital, The Fourth Military Medical University, Xi'an 710032, Shanxi,China
  • Received:2002-10-30 Published:2002-11-25 Online:2002-11-25

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摘要： 目的研究8Hz,90dB和130dB次声作用后不同时间点,大鼠延髓中星形胶质细胞和神经元的反应及其相互关系。方法8Hz,90dB和130dB次声作用于大鼠,2h/d,分别作用1、7、14、21、28天后采用免疫组织化学双标记方法,观察大鼠延髓中胶质源纤维酸性蛋白(GFAP)和Fos蛋白表达的情况。结果8Hz,130dB次声作用1d后大鼠延髓中开始出现GFAP阳性星形胶质细胞和Fos阳性神经元,分布相同,关系密切,并随作用次数增加而增多,14天后逐渐减少。90dB组大鼠各时间点GFAP反应均比130dB组弱。结论8Hz,90dB和130dB次声作用可以同时激活延髓星形胶质细胞和神经元。

关键词: 次声, 延髓, 胶质原纤维酸性蛋白(GFAP), Fos蛋白

Abstract: ObjectiveTo observe the relationship between astrocytes and neurons in rat's medulla oblongata after exposed to 8Hz, 90dB/130dB infrasound for different times. MethodsRats were exposed to 8Hz, 90dB/130dB infrasound for 2 hours per day.At the 1st,7th,12th,21th and 28th day, the expression of glial fibrillary acidic protein (GFAP) and Fos in medulla oblongata were detected using double-labling immunohistochemical method.Results GFAP-positive astrocytes and Fos-positive neurons were increased after exposure for one time. They showed similar topographic localization and formed close relationship. Their number increased as the exposure times increasing, and decreased gradually after 14 days. The reaction of 130dB group was stronger than that of 90dB group.Conclusions 8Hz, 90dB/130dB infrasound can activate both astrocytes and neurons in rats' medulla oblongata which might participate and regulate the response to infrasound.

Key words: infrasound, hippocampus, glial fibrillary acidic protein (GFAP), Fos