《中国康复理论与实践》

《中国康复理论与实践》 ›› 2021, Vol. 27 ›› Issue (8): 908-912.doi: 10.3969/j.issn.1006-9771.2021.08.005

• 基础研究 • 上一篇    下一篇

新生鼠缺氧缺血性脑损伤远期行为学研究

林爱金,王洁琼,敖丽娟(),陈茉弦()   

  1. 昆明医科大学康复学院,云南昆明市 650500
  • 收稿日期:2019-09-11 修回日期:2021-07-29 出版日期:2021-08-25 发布日期:2021-08-25
  • 通讯作者: 敖丽娟,陈茉弦 E-mail:13508710081@qq.com;37774290@qq.com
  • 作者简介:林爱金(1994-),女,汉族,福建福州市人,硕士,治疗师,主要研究方向：神经康复。
  • 基金资助:
    云南省教育厅科学研究基金项目(2019Y0346)

Long-term Behavioral Disorder after Hypoxic Ischemic Brain Damage in Newborn Mice

LIN Ai-jin,WANG Jie-qiong,AO Li-juan(),CHEN Mo-xian()   

  1. College of Rehabilitation, Kunming Medical University, Kunming, Yunnan 650500, China
  • Received:2019-09-11 Revised:2021-07-29 Published:2021-08-25 Online:2021-08-25
  • Contact: AO Li-juan,CHEN Mo-xian E-mail:13508710081@qq.com;37774290@qq.com
  • Supported by:
    Yunnan Education Department Scientific Research Fund(2019Y0346)

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摘要：

目的 观察新生鼠缺氧缺血性脑损伤(HIBD)后运动、情绪和学习记忆能力的远期变化。方法 10日龄C57BL/6小鼠50只分为对照组(n = 20)和HIBD组(n = 30)。HIBD组左侧颈总动脉结扎后缺氧45 min。2个月后,采用悬吊试验、明暗箱试验、高架十字迷宫试验、物体识别试验和Y迷宫试验进行评定。结果 最终19只小鼠造模成功。与对照组相比,HIBD组悬吊试验评分降低(t = 2.785, P < 0.05);明暗箱试验潜伏期显著延长(t = -4.320, P < 0.001),进入明箱的时间和次数减少(t > 2.603, P < 0.05);高架十字迷宫试验开臂停留时间显著缩短(t = 4.576, P < 0.001),闭臂停留时间明显延长(t = -3.287, P < 0.01);物体识别试验旧物体停留时间延长(t = -2.116, P < 0.05),物体识别指数下降(t = 2.823, P < 0.05);Y迷宫试验起始臂和新异臂停留时间缩短(t > 2.191, P < 0.05)。结论 新生鼠HIBD后远期行为变化表现为运动功能、学习记忆功能下降,出现焦虑行为。

关键词: 缺氧缺血性脑损伤, 运动, 认知, 焦虑, 小鼠

Abstract:

Objective To observe the long-term behavioral changes in movement, emotion, and learning and memory of newborn mice with hypoxic ischemic brain damage (HIBD). Methods A total of 50 ten-day old newborn C57BL/6 mice were divided into control group (n = 20) and HIBD group (n = 30). The left common carotid artery was ligated in HIBD group and stayed in anoxic chamber for 45 minutes. All the mice were tested with suspension test, light/dark box test, elevated plus maze test, object recognition test and Y maze test two months after surgery. Results There were 19 mice modeled successfully. Compared with the control group, the suspension test scores decreased (t = 2.785, P < 0.05); the time of latency of light/dark box test increased (t = -4.320, P < 0.001), the time and frequency in light box decreased (t > 2.603, P < 0.05); the time in open arm decreased (t = 4.576, P < 0.001) and the time in close arm increased (t = -3.287, P < 0.01) for the elevated plus maze test; the time nearing old object increased (t = -2.116, P < 0.05) and object recognition index decreased (t = 2.823, P < 0.05) for object recognition test; the time in the initial and novel arms decreased (t > 2.191, P < 0.05) for Y maze test in HIBD group. Conclusion The long-term disorders of behavior may include disabilities of motor, learning and memory, and disorder of anxiety, in newborn mice with HIBD.

Key words: hypoxic ischemic brain damage, motor, recognition, anxiety, mice