电针对血管性痴呆大鼠脑白质纤维和学习记忆功能的效果

doi:10.3969/j.issn.1006-9771.2020.03.011

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (3): 319-324.doi: 10.3969/j.issn.1006-9771.2020.03.011

电针对血管性痴呆大鼠脑白质纤维和学习记忆功能的效果

张冰雪¹,杨敏光¹,李建鸿¹,陶静^1,^2,³,梁胜祥²,柳维林¹,陈立典^1,^2,³()

1.福建中医药大学康复医学院,福建福州市 350122
2.福建中医药大学康复医疗技术国家地方联合工程研究中心,福建福州市 350122
3.福建省康复技术协同中心,福建福州市 350122

收稿日期:2019-09-27 修回日期:2020-01-20 出版日期:2020-03-25 发布日期:2020-04-01
通讯作者: 陈立典 E-mail:cld@fjtcm.edu.cn
作者简介:张冰雪(1995-),女,汉族,贵州六盘水市人,硕士研究生,主要研究方向：神经康复与认知科学。|陈立典(1963-),男,福建政和县人,博士,教授,主任医师,博士生导师,主要研究方向：神经康复及认知科学。
基金资助:
国家自然科学基金项目(81774424)

Effects of Electroacupuncture on White Matter Fibers and Learning-memory Function in Rats with Vascular Dementia

ZHANG Bing-xue¹,YANG Min-guang¹,LI Jian-hong¹,TAO Jing^1,^2,³,LIANG Sheng-xiang²,LIU Wei-lin¹,CHEN Li-dian^1,^2,³()

1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
2. National & Local Joint Engineering Research Center of Rehabilitation Medical Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
3. Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, Fujian 350122, China

Received:2019-09-27 Revised:2020-01-20 Published:2020-03-25 Online:2020-04-01
Contact: CHEN Li-dian E-mail:cld@fjtcm.edu.cn
Supported by:
National Natural Science Foundation of China(81774424)

摘要/Abstract

摘要：

目的观察电针百会、神庭对血管性痴呆(VD)大鼠脑白质纤维和学习记忆能力的影响。方法 Sprague-Dawley大鼠32只分离双侧颈总动脉,假手术组(n = 8)不结扎,其余造模成功后随机分为模型组(n = 8)、非穴组(n = 8)和电针组(n = 8)。电针组电针百会和神庭穴,非穴组刺激腋下非经非穴点,每天1次,共28 d。干预前后采用新物体识别试验进行评定,弥散张量成像观察大鼠脑白质纤维。结果干预前,与假手术组相比,模型组、非穴组和电针组新物体偏爱系数降低(P< 0.05),后三组间无显著性差异(P> 0.05);干预后,电针组新物体偏爱系数较模型组增高(P < 0.05)。干预前,与假手术组相比,模型组、非穴组和电针组胼胝体、扣带回、海马白质纤维各向异性分数(FA)降低;干预后,电针组海马、扣带回、胼胝体、外囊白质纤维FA较模型组增加。结论电针百会、神庭能改善VD大鼠记忆功能,可能与前额叶皮质、海马等脑区白质纤维修复有关。

关键词: 血管性痴呆, 电针, 白质纤维, 弥散张量成像, 学习记忆, 大鼠

Abstract:

Objective To observe the effect of electroacupuncture at Baihui (DU20) and Shenting (DU24) acupoints on white matter fiber and learning-memory function in rats with vascular dementia (VD). Methods Sprague-Dawley rats were randomly divided into operation group and sham group (n = 8). The operation group accepted two-vessel occlusion, while the sham group only separated the bilateral common carotid arteries without ligation. The rats modeled successfully were randomly divided into model group (n = 8), non-acupoint group (n = 8) and electroacupuncture group (n = 8). The electroacupuncture group accepted electroacupuncture at Baihui and Shenting, and the non-acupoint group accepted electroacupuncture at axillary non-acupoint, once a day for 28 days. All the rats were tested with object recognition test before and after intervention, while the white matter fibers were observed with Diffusion Tensor Imaging. Results Compared with the sham group, the preference coefficients of the model group, the non-acupoint group and the electroacupuncture group decreased before intervention (P< 0.05), and there was no significant difference among the later three groups (P> 0.05). The preference coefficients increased in the electroacupuncture group compared with the model group after intervention (P< 0.05). The fractional anisotropy (FA) of corpus callosum, cingulate gyrus and hippocampus decreased in the model group, the non-acupoint group and the electroacupuncture group compared with the sham group before intervention. FA of hippocampus, cingulate gyrus, corpus callosum and external capsule increased in the electroacupuncture group compared with the model group after intervention. Conclusion Electroacupuncture at Baihui and Shenting can improve learning-memory function of VD rats, which may be related with repairment of white matter fibers in prefrontal cortex, hippocampus and others.

Key words: vascular dementia, electroacupuncture, white matter fiber, diffusion tensor imaging, learning and memory, rats

中图分类号:

R749.1

张冰雪,杨敏光,李建鸿,陶静,梁胜祥,柳维林,陈立典. 电针对血管性痴呆大鼠脑白质纤维和学习记忆功能的效果[J]. 《中国康复理论与实践》, 2020, 26(3): 319-324.

ZHANG Bing-xue,YANG Min-guang,LI Jian-hong,TAO Jing,LIANG Sheng-xiang,LIU Wei-lin,CHEN Li-dian. Effects of Electroacupuncture on White Matter Fibers and Learning-memory Function in Rats with Vascular Dementia[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(3): 319-324.

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组别	n	β₁	β₂
假手术组	8	70.92±9.59	71.91±16.27
模型组	8	54.15±10.71^a	46.59±23.59^a
非穴组	8	52.77±11.14^a	47.86±18.86^a
电针组	8	57.03±10.31^a	46.73±12.84^a
F值		5.075	3.692
P值		0.006	0.023

组别	n	β₁	β₂
假手术组	8	78.38±12.85	61.68±6.82
模型组	8	53.27±12.51	46.63±12.66
非穴组	8	55.74±8.48	48.54±11.98
电针组	8	69.89±12.08^a	58.23±7.38^a
F值		8.390	4.236
P值		< 0.001	0.014

电针对血管性痴呆大鼠脑白质纤维和学习记忆功能的效果

Effects of Electroacupuncture on White Matter Fibers and Learning-memory Function in Rats with Vascular Dementia

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