《中国康复理论与实践》

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (4): 378-384.doi: 10.3969/j.issn.1006-9771.2018.04.002

• 专题:中枢神经损伤后水肿的机制及干预 • 上一篇    下一篇

大黄素对大鼠急性脊髓损伤后继发脊髓水肿的影响

曾欢欢, 黄英如, 李子健, 汪一, 张松   

  1. 1.重庆医科大学中医药学院针灸骨伤教研室,重庆市 400016;
    2.中医药防治代谢性疾病重庆市重点实验室,重庆市 400016
  • 收稿日期:2018-01-15 修回日期:2018-01-31 出版日期:2018-04-25 发布日期:2018-04-27
  • 通讯作者: 黄英如。E-mail: hyr12678@126.com
  • 作者简介:曾欢欢(1991-),女,汉族,重庆市人,硕士研究生,主要研究方向：脊髓损伤。
  • 基金资助:
    国家自然科学基金面上项目(No. 81373668; No. 81674002)

Effect of Emodin on Spinal Cord Edema Induced by Acute Spinal Cord Injury in Rats

ZENG Huan-huan, HUANG Ying-ru, LI Zi-jian, WANG Yi, ZHANG Song   

  1. 1. Department of Acupuncture and Orthopaedics of Traditional Chinese Medicine College, Chongqing Medical University, Chongqing 400016, China;
    2. Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing 400016, China
  • Received:2018-01-15 Revised:2018-01-31 Published:2018-04-25 Online:2018-04-27
  • Contact: HUANG Ying-ru. E-mail: hyr12678@126.com
  • Supported by:
    Supported by National Natural Science Foundation of China (General) (No. 81373668; No. 81674002)

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被引次数

2

摘要： 目的 探讨大黄素对大鼠急性脊髓损伤后脊髓水肿的影响及其机制。方法 将180只健康雌性Sprague-Dawley大鼠随机分为假手术组(A组),模型组(B组),大黄素低剂量组(C组)、中剂量组(D组)和高剂量组(E组),每组36只。采用Allen法制作大鼠脊髓损伤模型,术后3 d、7 d、14 d及28 d,BBB评分及斜板实验观察大鼠运动功能恢复。术后3 d,HE染色观察脊髓组织病理变化,干湿重法检测脊髓组织含水量,伊文思蓝(EB)染色检测血脊髓屏障(BSCB)通透性,RT-PCR和Western blotting检测水通道蛋白-4 (AQP-4)、基质金属蛋白酶-2 (MMP-2) mRNA和蛋白表达。结果 BBB评分及斜板实验结果显示,术后7 d、14 d、28 d,C组、D组和E组均优于B组(P<0.05),E组最佳(P<0.05)。术后3 d,HE染色显示,B组损伤节段内有大片出血灶,神经细胞肿胀、破坏,大量炎性细胞浸润,组织间隙增宽,水肿严重,C组、D组和E组上述病理改变改善,其中E组最明显;B组脊髓组织含水量较A组增高(P<0.05),D组、E组脊髓组织含水量低于B组和C组(P<0.05),E组低于D组(P<0.05);B组EB含量高于A组(P<0.05),C组、D组和E组EB含量均低于B组(P<0.05);C组、D组和E组AQP-4、MMP-2 mRNA和蛋白表达均低于B组(P<0.05),E组最低(P<0.05)。结论 大黄素能减轻大鼠急性脊髓损伤后的脊髓水肿,促进肢体功能康复,这可能与大黄素下调损伤后脊髓组织AQP-4和MMP-2表达,降低BSCB通透性有关。

关键词: 脊髓损伤, 水肿, 大黄素, 水通道蛋白-4, 基质金属蛋白酶-2, 大鼠

Abstract: Objective To investigate the effect of emodin on spinal cord edema induced by acute spinal cord injury (SCI) and its mechanism.Methods A total of 180 healthy female Sprague-Dawley rats were randomly divided into sham group (group A), model group (group B), and low-dose group (group C), middle-dose group (group D) and high-dose group (group E) of emodin, with 36 cases in each group. The SCI model was established with the modified Allen's method. Functional recovery was evaluated with Basso-Beattie-Bresnahan (BBB) score and inclined plate test three days, seven days, 14 days and 28 days after modeling. Three days after modeling, the pathological changes of the spinal cord were observed by HE staining; the water content of spinal cord was detected by dry-wet weight method, the blood-spinal cord barrier (BSCB) permeability was detected by Evans blue (EB) staining, and the expression of aquaporin-4 (AQP-4) and matrix metalloproteinase-2 (MMP-2) mRNA and protein were detected by RT-PCR and Western blotting respectively. Results The BBB score and inclined plate scores were better in groups C, D and E than in group B (P<0.05) seven days, 14 days and 28 days after modeling, especially in group E (P<0.05). Three days after modeling, HE staining showed that there was a large hemorrhage in the section of group B, the nerve cells were swollen and damaged, and a large number of inflammatory cells were infiltrated, the tissue gap was widened and the edema was severe. The above pathological changes were better in groups C, D and E than in group B, especially in group E. The spinal cord water content was higher in group B than in group A (P<0.05), and was lower in groups D and E than in groups B and C (P<0.05). EB content was higher in group B than in group A (P<0.05), and was lower in groups C, D and E than in group B (P<0.05). The expression of AQP-4, MMP-2 mRNA and protein were lower in groups C, D and E than in group B (P<0.05), especially in group E (P<0.05). Conclusion Emodin can alleviate spinal cord edema, and improve hind limb movement function after SCI, which could be related with the down-regulation of AQP-4 and MMP-2 expression, and the reduction of the permeability of BSCB.

Key words: spinal cord injury, edema, emodin, aquaporin-4, matrix metalloproteinase-2, rats

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