丰富环境对血管性痴呆大鼠侧脑室室管膜下区神经再生的影响

doi:10.3969/j.issn.1006-9771.2017.12.004

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (12): 1384-1389.doi: 10.3969/j.issn.1006-9771.2017.12.004

丰富环境对血管性痴呆大鼠侧脑室室管膜下区神经再生的影响

贺旭^1,2,3, 刘英飞⁴, 罗明英⁵, 成绍武^2,3, 葛金文^2,3

1.益阳医学高等专科学校解剖教研室,湖南益阳市 413000;
2.湖南中医药大学中西医结合学院,湖南长沙市 410208;
3.中西医结合心脑疾病防治湖南省重点实验室,湖南长沙市 410208;
4.益阳医学高等专科学校附属医院内一科,湖南益阳市 413000;
5.昆明医科大学人体解剖教研室,云南昆明市 650500。

收稿日期:2017-06-30 修回日期:2017-08-17 出版日期:2017-12-25 发布日期:2017-12-28
通讯作者: 葛金文(1965-),男,汉族,湖南娄底市人,博士,教授,博士生导师,主要研究方向：脑血管病发病机制及中西医结合防治研究和中西医结合方法学研究。E-mail: 40831556@qq.com。
作者简介:贺旭(1984-),男,汉族,湖南邵阳市人,博士,讲师,主要研究方向：中药在心脑血管疾病神经发生与神经再生的作用。
基金资助:
1.国家自然科学基金项目(No.81500377); 2.湖南省教育厅优秀青年课题(No.16B269); 3.益阳医学高等专科学校引进高层次人才科研启动费项目(No.2016-001)

Effect of Enriched Environment on Neuroregeneration in Subventricle Zone of Rats with Vascular Dementia

HE Xu^1,2,3, LIU Ying-fei⁴, LUO Ming-ying⁵, CHENG Shao-wu^2,3, GE Jin-wen^2,3

1.Department of Anatomy, Yiyang Medical College, Yiyang, Hunan 413000, China;
2.Department of Integrated Traditional and Western Medicine, Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, China;
3.Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Disease, Changsha, Hunan 410208, China;
4.Hospital Affiliated to Yiyang Medical College, Yiyang, Hunan 413000, China;
5.Department of Human Anatomy, Kunming Medical College, Kunming, Yunnan 650500, China

Received:2017-06-30 Revised:2017-08-17 Published:2017-12-25 Online:2017-12-28
Contact: GE Jin-wen. E-mail: 40831556@qq.com

摘要/Abstract

摘要： 目的探讨丰富环境(EE)对血管性痴呆大鼠侧脑室室管膜下区(SVZ)神经再生的影响。方法 29只大鼠分成假手术组(n=5)、血管性痴呆组(VD组,n=12)和丰富环境组(EE组,n=12)。采用两血管阻断法(2-VO)制作血管性痴呆模型。VD组和假手术组常规饲养条件下饲养,EE组给予丰富环境干预。30 d后免疫组织化学染色观察SVZ微管相关蛋白Doublecortin (DCX)与外源性细胞增殖标记物BrdU的表达,免疫荧光双标观察SVZ DCX/Ki67、DCX/p-cAMP反应元件结合蛋白(CREB)的共表达情况。结果与假手术组相比,VD组BrdU⁺细胞数目(t=2.989, P=0.026)、DCX⁺细胞平均光密度值(t=3.069, P=0.005)增加;与VD组比较,EE组BrdU⁺细胞数目(t=3.067, P=0.027)、DCX⁺细胞平均光密度值(t=2.907, P=0.011)进一步增加。EE组DCX/Ki67 (t=2.994, P=0.040)、DCX/p-CREB(t=4.707, P=0.009)共表达细胞高于VD组。结论丰富环境可通过CREB信号通路上调血管性痴呆大鼠SVZ神经再生水平。

关键词: 血管性痴呆, 丰富环境, 室管膜下区, 神经再生, 大鼠

Abstract: Objective To explore the effect of enriched environment on neuroregeneration in subventricle zone (SVZ) of rats with vascular dementia. Methods All rats were divided into sham group (n=5), vascular dementia group (VD group, n=12) and enriched environment group (EE group, n=12). Then, 2-VO method was applied to make the vascular dementia model. The sham group and VD group received conventional breeding environment for 30 days, while EE group was subjected to enriched environment for 30 days. Immunohistochemistry was applied to detect the BrdU and Doublecortin (DCX) expression in SVZ, and DCX/Ki67, DCX/p-cAMP-response element binding protein (CREB) expression in SVZ was assessed by immunofluorescence double-labeling technique. Results Compared with the sham group, the number of DCX⁺ cells (t=2.989, P=0.026) and BrdU⁺ cells (t=3.069, P=0.005) increased in VD group, and increased more in EE group (t=3.067, P=0.027; t=2.907, P=0.011). Besides, the number of the DCX/Ki67 (t=2.994, P=0.040) and DCX/p-CREB (t=4.707, P=0.009) cells was significantly higher in EE group than in VD group. Conclusion Enriched environment could up-regulate the neuroregeneration capacity in SVZ of rats with vascular dementia through CREB signal pathway.

Key words: vascular dementia, enriched environment, subventricle zone, neuroregeneration, rats

中图分类号:

R749.1

贺旭, 刘英飞, 罗明英, 成绍武, 葛金文. 丰富环境对血管性痴呆大鼠侧脑室室管膜下区神经再生的影响[J]. 《中国康复理论与实践》, 2017, 23(12): 1384-1389.

HE Xu, LIU Ying-fei, LUO Ming-ying, CHENG Shao-wu, GE Jin-wen. Effect of Enriched Environment on Neuroregeneration in Subventricle Zone of Rats with Vascular Dementia[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(12): 1384-1389.

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丰富环境对血管性痴呆大鼠侧脑室室管膜下区神经再生的影响

Effect of Enriched Environment on Neuroregeneration in Subventricle Zone of Rats with Vascular Dementia

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