跑台运动对脑外伤大鼠基质金属蛋白酶-2表达的影响

doi:10.3969/j.issn.1006-9771.2017.05.007

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (5): 525-528.doi: 10.3969/j.issn.1006-9771.2017.05.007

跑台运动对脑外伤大鼠基质金属蛋白酶-2表达的影响

沈夏锋¹, 田闪², 吴毅², 朱玉连²

1.上海市第一康复医院康复科,上海市 200092;
2.复旦大学附属华山医院康复医学科,上海市 200040。

收稿日期:2017-03-08 修回日期:2017-04-11 出版日期:2017-05-25 发布日期:2017-05-24
通讯作者: 朱玉连。E-mail: hsyykfkzyl@163.com。
作者简介:沈夏锋(1970-),男,汉族,浙江苍南县人,副主任医师,主要研究方向：神经康复。
基金资助:
杨浦区科学技术委员会、卫生和计划生育委员会课题(No.YP15M13)

Effect of Treadmill Exercise on Expression of Matrix Metalloproteinase-2 in Rats with Traumatic Brain Injury

SHEN Xia-feng¹, TIAN Shan², WU Yi², ZHU Yu-lian²

1. Department of Rehabilitation Medicine, The First Rehabilitaion Hospital of Shanghai, Shanghai 200092, China;
2. Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China

Received:2017-03-08 Revised:2017-04-11 Published:2017-05-25 Online:2017-05-24
Contact: ZHU Yu-lian. E-mail: hsyykfkzyl@163.com

摘要/Abstract

摘要： 目的观察跑台训练对脑外伤大鼠基质金属蛋白酶-2 (MMP-2)表达的影响。方法 55只成年Sprague-Dawley大鼠随机分为假手术组(n=15)、对照组(n=20)和运动组(n=20),后两组复制中重度脑外伤模型,运动组进行电动跑台训练1周。术后6、12、18、24 d,采用足误试验评定运动协调功能;术后48 h,采用伊文思蓝(EB)染色检测大鼠血脑屏障通透性;术后7 d,Western blotting检测MMP-2的表达。结果运动组在术后6 d评分明显降低,但随后增高,其中12 d、18 d及24 d评分比对照组高(F>4.793, P<0.05);运动组EB渗出量显著减少(t=-8.091, P<0.001);运动组MMP-2蛋白表达显著下降(t=-13.12, P<0.001)。结论大鼠脑外伤后早期进行跑台训练,可改善运动功能,可能与抑制MMP-2,从而减轻血脑屏障破坏有关。

关键词: 脑外伤, 跑台训练, 血脑屏障, 基质金属蛋白酶, 大鼠

Abstract: Objective To determine the effect of treadmill exercise on expression of matrix metalloproteinase-2 (MMP-2) in rats following traumatic brain injury (TBI). Methods Fifty-five male adult Sprague-Dawley rats were randomly assigned to sham (n=15), control (n=20) and exercise (n=20) groups, the later two groups subjected to unilateral cortical contusion injury (CCI). All the rats were assessed with foot-fault test 6, 12, 18 and 24 days after CCI. Evans blue perfusion was used to evaluate the integrity of the blood-brain-barrier (BBB) 48 hours after CCI. Protein expression of MMP-2 was determined with Western blotting one week after CCI. Results The score of foot-fault test improved more in the exercise group than in the control group 12, 18 and 24 days after CCI (F>4.793, P<0.05). Evans blue extravasation was less in the exercise group than in the control group (t=-8.091, P<0.001), as well as the expression of MMP-2 (t=-13.12, P<0.001). Conclusion Early treadmill exercise can improve the motor function in rats with TBI, which may associate with inhibition of MMP-2 expression to protect BBB integrity.

Key words: traumatic brain injury, treadmill exercise, blood-brain-barrier, matrix metalloproteinase, rats

中图分类号:

R651.1

沈夏锋, 田闪, 吴毅, 朱玉连. 跑台运动对脑外伤大鼠基质金属蛋白酶-2表达的影响[J]. 《中国康复理论与实践》, 2017, 23(5): 525-528.

SHEN Xia-feng, TIAN Shan, WU Yi, ZHU Yu-lian. Effect of Treadmill Exercise on Expression of Matrix Metalloproteinase-2 in Rats with Traumatic Brain Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(5): 525-528.

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跑台运动对脑外伤大鼠基质金属蛋白酶-2表达的影响

Effect of Treadmill Exercise on Expression of Matrix Metalloproteinase-2 in Rats with Traumatic Brain Injury

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