《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (01): 1-2.

• 专题研究 •    下一篇

血管内皮细胞生长因子在烧伤后肉芽组织和增生性瘢痕中的表达

沈锐1; 利天增1; 祁少海1; 李叶扬2; 谢举临1; 徐盈斌1; 赵继宗3   

  1. 1.中山医科大学附属第一医院烧伤科 广东广州市 510080;2.广州市红十字会医院烧伤整形科 广东广州市 510050;3.中 山医科大学外科实验室 广东广州市 510089
  • 收稿日期:2001-08-15 出版日期:2002-01-25 发布日期:2002-01-25

Study of expression of vascular endothelial growth factor in granulation tissue of burn wound and post-burn hypertrophic scar at excessive stages

SHEN Rui,LI Tian-zeng,QI Shao-hai,et al   

  1. Department of Burn, First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, Guangdong, China
  • Received:2001-08-15 Published:2002-01-25 Online:2002-01-25

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摘要： 目的观察血管内皮细胞生长因子(VEGF)在烧伤创面肉芽组织和烧伤后不同时期增生性瘢痕中的动态变化,探讨VEGF在烧伤创面修复过程中的表达规律及其作用。方法选择临床手术切除的新鲜烧伤创面肉芽及各个时期的增生性瘢痕,制成匀浆液,应用ELISA方法检测匀浆液中VEGF的变化。结果VEGF可在烧伤创面肉芽组织中有一定的表达,随着增生性瘢痕的发展,其表达量进一步增加,在4—6个月的增生性瘢痕中,表达量达到峰值。瘢痕成熟后,VEGF的组织表达逐渐下降。结论VEGF的表达量与增生性瘢痕组织中的血管丰富程度以及成熟程度密切相关。

关键词: 增生性瘢痕, 血管内皮生长因子, 组织匀浆液, 表达

Abstract: ObjectiveTo explore the role of vascular endothelial growth factor(VEGF) in the growth and development of hypertrophic scar.MethodsThe burn wound samples of various stages were selected from transition of wound granulation tissue to scar and in long-persisting post-burn hypertrophic scar, and the concentrations of VEGF protein were detected using enzyme-linked immunosorbent essay (ELISA) method. ResultsThe tissue homogenate concentration of VEGF protein increases gradually from the wound granulation tissue to hypertrophic scar before it achieves summit concentration during 4 to 6 month. The concentration of VEGF degreases gradually after the maturation of hypertrophic scar. The high concentration of VEGF is synonymous with the large amount of capillary of the immature scar.ConclusionsThe abnormal expression of VEGF is related to the growth and development of hypertrophic scar and induces excessive and uncontrollable angiogenesis.

Key words: hypertrophic scar, vascular endothelia growth factor(VEGF), tissue homogenate, expression