《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

电针预处理对大鼠脑缺血再灌注损伤后炎性因子及细胞凋亡的影响①

朱路文1，叶涛2，姜云飞2，吴孝军2，李宏玉2，宋名扬2，唐强1   

  1. 1. 黑龙江中医药大学附属第二医院，黑龙江哈尔滨市150001；2. 黑龙江中医药大学，黑龙江哈尔滨市150040。
  • 出版日期:2016-07-25 发布日期:2016-09-22

Effects of Electro-acupuncture Pretreatment on Interleukin-1β, Interleukin-6 and Apoptosis in Rats after Cerebral Ischemia-reperfusion Injury

ZHU Lu-wen1, YE Tao2, JIANG Yun-fei2, WU Xiao-jun2, LI Hong-yu2, SONG Ming-yang2, TANG Qiang1   

  1. 1. The Second Hospital Affiliated to Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China; 2. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China
  • Published:2016-07-25 Online:2016-09-22

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摘要： 目的探讨电针预处理对大鼠脑缺血再灌注损伤后白细胞介素(IL)-1β、IL-6 含量及细胞凋亡的影响。方法36 只清洁级雄性Sprague-Dawley 大鼠按随机数字表法分为假手术组、模型组和电针预处理组，每组12 只。大脑中动脉阻塞(MCAO)建立脑缺血2 h 再灌注模型。再灌注后24 h，酶联免疫吸附法检测血清、脑组织IL-1β、IL-6 的含量，TUNEL染色检测缺血半暗区神经细胞凋亡。结果与模型组比较，电针预处理组大鼠血清、脑组织IL-1β、IL-6 的含量明显下降(P<0.01)，TUNEL阳性细胞数显著下降(P<0.001)。结论电针预处理降低炎症反应，减少细胞凋亡。

关键词: 脑缺血再灌注；电针；预处理；白细胞介素-1&beta, ；白细胞介素-6；细胞凋亡；炎症反应；大鼠

Abstract: Objective To investigate the effect of electro-acupuncture pretreatment on content of interleukin (IL) -1β and IL-6 in serum and ischemic penumbra, and apoptosis in ischemic penumbra in rats after cerebral ischemia- reperfusion injury. Methods Thirty- six male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12), and electro-acupuncture pretreatment group (n= 12). The middle cerebral arteries were occluded for 120 minutes and reperfused. Twenty-four hours after reperfusion, the level of IL-1β and IL-6 in serum and brain tissue was detected with enzyme-linked immunosorbent, and apoptosis of ischemic penumbra was detected with TUNEL. Results The content of IL-1β and IL-6 in the serum and brain tissue, and the number of TUNEL-positive cells decreased in the electro- acupuncture pretreatment group compared with that in the model group (P<0.01). Conclusion Electro-acupuncture pretreatment may inhibit inflammatory response and apoptosis in cerebral ischemia-reperfusion injury.

Key words: cerebral ischemia- reperfusion, electro- acupuncture, pretreatment, interleukin- 1β, interleukin- 6, inflammatory response, apoptosis, rats