《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

有氧运动预处理对全脑缺血再灌注大鼠再生相关因子的影响①

孙竹梅1，李建民1，王国立1，赵雅宁1，陈长香1，赵旭1，王静2   

  1. 1. 华北理工大学临床医学院，河北唐山市063000；2. 唐山市开滦总医院，河北唐山市063000。
  • 出版日期:2016-07-25 发布日期:2016-09-22

Effect of Aerobic Exercise Preconditioning on Growth-associated Protein-43 and Nogo-A in Rats after Cerebral Ischemia/Reperfusion

SUN Zhu-mei1, LI Jian-min1, WANG Guo-li1, ZHAO Ya-ning1, CHEN Chang-xiang1, ZHAO Xu1, WANG Jing2   

  1. 1. North China University of Science and Technology, Tangshan, Hebei 063000, China; 2. Kailuan General Hospital, Tangshan, Hebei 063000, China
  • Published:2016-07-25 Online:2016-09-22

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摘要： 目的探讨有氧运动预处理对脑缺血大鼠脑组织海马区生长相关蛋白-43 (GAP-43)、Nogo-A的影响。方法120 只Sprague-Dawley 大鼠平均分为假手术组、脑缺血再灌注组和有氧运动预处理组。改良Pulsinelli 四血管阻断(4-VO)法制备脑缺血再灌注模型。分别在缺血后6 h、1 d、3 d、7 d 各取5 只大鼠，HE染色观察大鼠海马组织神经细胞形态变化，免疫组化法检测海马组织GAP-43、Nogo-A表达；另5 只大鼠RT-PCR法检测GAP-43、Nogo-A水平。结果与脑缺血再灌注组相比，有氧运动预处理组的神经元密度、GAP-43 蛋白和mRNA表达水平明显升高(P<0.01)，Nogo-A蛋白和mRNA表达水平明显降低(P<0.01)。结论有氧运动预处理可促进脑缺血再灌注后神经元细胞存活和轴突再生，与上调脑组织海马区GAP-43表达并下调Nogo-A表达有关。

关键词: 脑缺血再灌注, 有氧运动, 生长相关蛋白-43, Nogo-A, 大鼠

Abstract: Objective To observe the effect of aerobic exercise preconditioning on growth-associated protein-43 (GAP-43) and Nogo-A in rats after cerebral ischemia/reperfusion. Methods Sprague-Dawley rats were equally divided into sham group (n=40), cerebral ischemia/ reperfusion group (n=40) and aerobic exercise preconditioning group (n=40), and global cerebral ischemia model was formed with modified four-vessel occlusion. The rats was sacrificed six hours, one day, three days and seven days after ischemia, respectively. The hippocampus neural cells were observed in five rats with HE staining and immunohistochemistry of GAP-43 and Nogo-A, and the other five rats were tested with RT-PCR of GAP-43 and Nogo-A. Results Compared with those in the cerebral ischemia/reperfusion group, the apoptotic neurons and expression of GAP-43 significantly increased all the time points in the aerobic exercise preconditioning group (P<0.01), while the expression of Nogo-A decreased (P<0.01). Conclusion Aerobic exercise preconditioning can promote the regeneration of neuronal cells and axon after cerebral ischemia/reperfusion injury, which is related to the regulation of GAP-43 and Nogo-A.

Key words: cerebral ischemia/reperfusion, aerobic exercise, growth-associated protein-43, Nogo-A, rats