《中国康复理论与实践》

《中国康复理论与实践》

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乙酰左旋肉碱对急性脊髓损伤大鼠细胞自噬、凋亡及运动功能的影响①

孟庆峰1,2，张明超1，卢伟1，毕云龙1，范仲凯1   

  1. 1. 辽宁医学院附属第一医院骨科，辽宁锦州市121001；2. 抚顺市中心医院骨科，辽宁抚顺市113006。
  • 出版日期:2016-07-25 发布日期:2016-09-22

Effects of Acetyl-l-carnitine on Autophagy, Apoptosis, and Locomotor Function after Acute Spinal Cord Injury in Rats

MENG Qing-feng1,2, ZHANG Ming-chao1, LU Wei1, BI Yun-long1, FAN Zhong-kai1   

  1. 1. Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou, Liaoning 121001, China；2. Department of Orthopedics, Fushun Central Hospital, Fushun, Liaoning 113006, China
  • Published:2016-07-25 Online:2016-09-22

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摘要： 目的旨在检测乙酰左旋肉碱(ALC)对大鼠急性脊髓损伤(SCI)后微管相关蛋白1 轻链3(LC3)-Ⅱ、细胞凋亡及运动功能的影响。方法成年雌性Sprague-Dawley 大鼠36 只随机分为假手术组(Sham 组)、单纯脊髓损伤组(SCI 组)、ALC治疗组，每组12 只。Allen 法建立大鼠T10脊髓损伤模型。损伤后3 d 行BBB运动评分；取脊髓组织，Western blotting 和免疫荧光标记技术检测LC3-Ⅱ表达，TUNEL 法观察细胞凋亡。结果与Sham 组相比，SCI 组LC3-Ⅱ明显升高(P<0.01)，细胞凋亡显著增加(P<0.001)，BBB评分显著降低(P<0.001)；与SCI组相比，ALC组LC3-Ⅱ显著升高(P<0.001)，细胞凋亡显著降低(P<0.001)，BBB评分明显提高(P<0.01)。结论ALC可能通过增强大鼠脊髓损伤后细胞自噬，减少细胞凋亡，促进大鼠运动功能恢复。

关键词: 急性脊髓损伤, 乙酰左旋肉碱, 自噬, 凋亡, 大鼠

Abstract: Objective To observe the effects of acetyl-l-carnitine (ALC) on autophagy, apoptosis and motor function after acute spinal cord injury (ASCI) in rats. Methods Thirty-six adult female Sprague-Dawley rats were randomly divided into sham operation group (Sham group, n=12), simple spinal cord injury group (SCI group, n=12), ALC treatment group (ALC group, n=12). Spinal cord injury model at the level of T10 segment was established using Allen's method. They were assessed with Basso-Beattle-Bresnahan (BBB) scale three days after injury. Then the rats were sacrificed, and the expression of microtubule associated protein 1 light chain 3 (LC3) -II in spinal cord was detected with Western blotting and immunofluorescent labeling, and the number of apoptotic cells were assessed with TUNEL staining. Results The expression of LC3-II and the number of apoptotic cells increased in SCI group compared with those in Sham group (P<0.01), while the BBB score decreased (P<0.001). The expression of LC3-II increased and the number of apoptotic cells decreased in ALC group compared with those in SCI group (P<0.001), while the BBB score increased (P<0.01). Conclusion ALC may promote autophagy, and inhibit apoptosis to improve the locomotor function after ASCI.

Key words: acute spinal cord injury, acetyl-l-carnitine, autophagy, apoptosis, rats