《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (09): 1025-1030.

• 基础研究 • 上一篇    下一篇

血管性痴呆大鼠海马齿状回神经前体细胞增殖的动态变化及养血清脑颗粒的影响

李晶，刘颖，刘斌，罗永伟，范颖，马原源，毛文静，李世英   

  1. 华北理工大学附属医院神经内一科，河北唐山市 063000。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-09-25 发布日期:2015-09-25

Proliferation of Neural Precursor Cells in Dentate Gyrus of Hippocampus and Effect of Yangxue Qingnao Granule in Vascular Dementia Rats

LI Jing, LIU Ying, LIU Bin, LUO Yong-wei, FAN Ying, MA Yuan-yuan, MAO Wen-jing, LI Shi-ying   

  1. First Department of Neurology, Hospital Affiliated to North China University of Science and Technology, Tangshan, Hebei 063000, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-09-25 Online:2015-09-25

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摘要： 目的 观察血管性痴呆(VD)大鼠海马齿状回(DG)神经前体细胞(NPCs)增殖的动态变化及养血清脑颗粒的影响。方法 72只 Sprague-Dawley大鼠随机分为假手术组(n=24)、VD模型组(模型组, n=24)和养血清脑颗粒治疗组(治疗组, n=24)，采用改良的 Pulsinelli四血管阻断法制作 VD大鼠模型。分别在造模术后 1、2、4和 8周采用 Western blotting法检测 Nestin的表达水平，用免疫荧光法观察海马齿状回区5-溴脱氧尿嘧啶(BrdU)和BrdU/Nestin的表达。结果 模型组和治疗组各时间点大鼠Nestin、BrdU和BrdU/Nestin 表达持续增多，4 周时达高峰。与假手术组比较，模型组各时间点 Nestin、BrdU 和 BrdU/Nestin 表达明显增多(P<0.01)；与模型组比较，治疗组各时间点 Nestin、BrdU和 BrdU/Nestin表达明显增多(P<0.01)。结论 养血清脑颗粒可促进 VD大鼠海马齿状回区NPCs增殖。

关键词: 血管性痴呆, 神经前体细胞, 增殖, 齿状回, 海马, 养血清脑颗粒

Abstract: Objective To observe dynamic variation of neural precursor cells in dentate gyrus of hippocampus and effect of Yangxue Qingnao Granule on it in vascular dementia rats. Methods 72 Sprague-Dawley rats were randomly divided into sham group (n=24), vascular dementia group (model group, n=24) and Yangxue Qingnao Granule group (treatment group, n=24). The vascular dementia model was established with modified Pulsineli's four-vessel occlusion. The expression of Nestin was detected with Western blotting, the expression of 5-bromodeoxyuridine (BrdU) and BrdU/Nestin were detected with immunofluorescence in dentate gyrus of hippocampus 1, 2, 4 and 8 weeks after modeling. Results The expression of Nestin, BrdU and BrdU/Nestin increased in the model and treatment groups with time, peaked at 4 weeks after modeling, and it was more than that of the sham group on all the time points (P<0.01). However, it was more in the treatment group than in the model group on all the time points (P<0.01). Conclusion Yangxue Qingnao Granule promotes the proliferation of neural precursor cells in dentate gyrus of hippocampus in vascular dementia rats.

Key words: vascular dementia, neural precursor cells, proliferation, dentate gyrus, hippocampus, Yangxue Qingnao Granule