《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (09): 1012-1015.

• 基础研究 • 上一篇    下一篇

电刺激小脑顶核对脑缺血再灌注后大鼠脑组织损伤的影响及其机制

何兰英 1，罗勇 2,3，王健 1，董为伟 2,3   

  1. 1.成都市第二人民医院神经内科，四川成都市 610017； 2. 重庆医科大学附属第一医院神经内科，重庆市 400016；3.重庆市神经病学重点实验室，重庆市 400016。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-09-25 发布日期:2015-09-25

Effect of Electrical Stimulation to Cerebellar Fastigial Nucleus on Cerebral Ischemia-reperfusion Injury in Rats

HE Lan-ying1, LUO Yong2,3, WANG Jian1, DONG Wei-wei2,3   

  1. 1. Department of Neurology, The Second People's Hospital of Chengdu City, Chengdu, Sichuan 610017; 2. Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; 3. Chongqing Key Laboratory of Neurology, Chongqing 400016, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-09-25 Online:2015-09-25

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摘要： 目的 探讨电刺激小脑顶核对大鼠局灶脑缺血再灌注后脑保护作用的机制。方法 Sprague-Dawley大鼠分为正常对照组(NC组)、缺血再灌注组(I/R组)、缺血再灌注后小脑顶核刺激组(FNS组)、毁损小脑顶核组(FNL组)，后3组根据再灌注时间分为7 d和14 d两个亚组，每个亚组6只。大脑中动脉线栓法制作缺血再灌注模型。相应时间点采用Western blotting检测脑梗死周围组织核因子-κB (NF-κB) P50蛋白表达，逆转录-聚合酶链反应法(RT-PCR)检测肿瘤坏死因子-α (TNF-α)和Bcl-xL mRNA表达，同时检测各组脑梗死体积。结果 与 I/R组比较，FNS组 NF-κB P50蛋白表达各时间点均增高(P<0.05)，TNF-α mRNA表达明显降低(P<0.01)，Bcl-xL mRNA表达升高(P<0.05)，梗死面积明显减少(P<0.01)。与I/R组比较，FNL组上述指标均无显著性差异(P>0.05)。结论 FNS可有效提高脑缺血再灌注后NF-κB P50蛋白、Bcl-xL mRNA表达，抑制下游炎症因子TNF-α mRNA的表达，减小脑梗死体积，可能是FNS发挥中枢神经保护的机制之一。

关键词: 脑缺血再灌注损伤, 电刺激, 小脑顶核, 核因子-κB, 肿瘤坏死因子-α, Bcl-xL, 大鼠

Abstract: Objective To investigate the effect of electrical stimulation to cerebellar fastigial nucleus on expression of nuclear factor-kappa B (NF-кB) P50, tumor necrosis factor-α (TNF-α) and Bcl-xL mRNA in rats brain after cerebral ischemia-reperfusion. Methods Sprague-Dawley rats were randomly divided into normal control group (NC group), cerebral ischemia-reperfusion group (I/R group), fastigial nucleus stimulation (FNS) group, and fastigial nucleus lesion (FNL) group. A focal cerebral ischemia-reperfusion model was established with middle cerebral artery occlusion (MCAO). 7 and 14 days after operation, the infarct volume was measured, and the protein of NF-кB P50 in rats brain was detected with Western blotting; the expression of TNF-α and Bcl-xL mRNA was detected with RT-PCR. Results Compared with I/R group, the expression of NF-кB P50 protein increased in FNS group (P<0.05), with the decrease of expression of TNF-α mRNA (P<0.01) and increase of Bcl-xL mRNA (P<0.05), while the infarct size decreased (P<0.01). There was no significant difference between FNL group and I/R group for all the measurements (P>0.05). Conclusion FNS could induce the expression of P50 protein and Bcl-xL mRNA, and inhibit the expression of TNF-α mRNA, and reduce infarct size, which may associated with the neuroprotection of central nervous system from injury.

Key words: cerebral ischemia-reperfusion, electrical stimulation, cerebellar fastigial nucleus, focal factor-kappa B, tumor necrosis factor-α, Bcl-xL, rats