《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (08): 900-904.

• 特稿 • 上一篇    下一篇

电针曲池、足三里穴对缺血再灌注损伤大鼠线粒体Caspase-3 途径诱导细胞凋亡的影响

上官豪1，柳维林1，陈文列2，郑薏1，王鲜1，林云娇1，王露露1，陈立典1，陶静1   

  1. 1.福建中医药大学康复医学院，福建福州市350122；2.福建中医药大学中西医结合研究院，福建福州市350122。作者简介：上官豪(1990-)，男，汉族，福建清流县人，硕士研究生，主要研究方向：脑血管病的临床与康复。通讯作者：陶静(1977-)，女，汉族，江苏淮安市人，医学博士，副教授，硕士研究生导师，主要研究方向：脑血管病的临床与康复。E-mail: taojing01@163.com。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-08-25 发布日期:2015-08-25

Effects of Electroacupuncture at Quchi (LI11) and Zusanli (ST36) on Neuronal Apoptosis Induced by Mitochondria- caspase-3 Pathway in Rats with Cerebral Ischemia-reperfusion Injury

SHANGGUAN Hao1, LIU Wei-lin1, CHEN Wen-lie2, ZHENG Yi1, WANG Xian1, LIN Yun-jiao1, WANG Lu-lu1, CHEN Li-dian1, TAO Jing1   

  1. 1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China; 2. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-08-25 Online:2015-08-25

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摘要： 目的观察电针曲池、足三里穴对脑缺血再灌注损伤大鼠缺血周围区皮质神经细胞超微结构和caspase-3、Bcl-2、Bax蛋白表达的影响。方法36 只雄性Sprague-Dawley 大鼠随机分为假手术组、模型组及电针组，每组12 只。采用Longa 线栓法制作左侧大脑中动脉缺血再灌注损伤模型。电针偏瘫侧曲池、足三里穴30 min。治疗前后分别进行神经行为学评分，采用透射电镜观察神经细胞超微结构，Western blotting 法检测caspase-3、Bcl-2 及Bax 的表达。结果电针治疗后，电针组大鼠神经行为学评分较模型组降低(P<0.05)；电镜下电针组较模型组核染色质均匀，线粒体数量增加；电针组Bcl-2 蛋白表达水平明显高于模型组(P<0.01)，caspase-3、Bax蛋白表达低于模型组(P<0.05)。结论电针曲池、足三里穴可通过线粒体-caspase-3 途径抑制缺血周围区皮质神经细胞凋亡。

关键词: 脑缺血再灌注, 电针, 线粒体, caspase-3, 凋亡, 大鼠

Abstract: Objective To investigate the effects of electroacupuncture (EA) at Quchi (LI11) and Zusanli (ST36) acupoints on the ultrastructural structure of cortical neurons in peripheral area and the protein expression of caspase- 3, Bcl- 2, Bax in rats with cerebral ischemia- reperfusion injury. Methods 36 male adult Sprague-Dawley rats were randomly divided into sham operation group, model group, and electroacupuncture group, with 12 rats in each group. The model group and electroacupuncture group were performed with left middle cerebral artery occlusion (MCAO) according to the modified Longa' methods. The electroacupuncture group received electroacupuncture at Quchi (LI11) and Zusanli (ST36) on the paralyzed limb, for 30 minute. The neurobehavioral scores were recorded before and after treatment. The ultrastructural structure of cortical neurons was observed with transmission electron microscope (TEM). The protein expression of caspase- 3, Bcl-2 and Bax were detected by Western blotting technique. Results The neurobehavioral score was lower in the electroacupuncture group than in the control group (P<0.05). Compared with the model group, the chromatin of neurons was even relatively, and the number of mitochondria increased. The expression of Bcl-2 was higher and the expression of caspase-3 and Bax was lower in the electroacupuncture group than in the model group (P<0.05). Conclusion Electroacupuncture at Quchi (LI11) and Zusanli (ST36) acupoints can inhibit the neurons apoptosis in peripheral area through mitochondria-caspase-3 pathway.

Key words: cerebral ischemia-reperfusion, electroacupuncture, mitochondria, caspase-3, apoptosis, rats