《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (07): 770-772.

• 特稿 • 上一篇    下一篇

谷红注射液对脑缺血再灌注大鼠皮层血管内皮生长因子表达的影响

张蕊，梁珍，杨楠，纪超，刘雁勇，左萍萍   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-07-25 发布日期:2015-07-25

Effects of Guhong Injection on Expression of Vascular Endothelial Growth Factor in Cortex after Cerebral Ischemia-reperfusion in Rats

ZHANG Rui, LIANG Zhen, YANG Nan, JI Chao, LIU Yan-yong, ZUO Ping-ping   

  1. Institute of Basic Medical Sciences, Chinese Academy of Medical Science, Beijing 100005, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-07-25 Online:2015-07-25

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摘要： 目的 探讨谷红注射液对脑缺血再灌注大鼠梗死侧皮层区血管内皮生长因子(VEGF)表达的影响。方法 健康成年雄性 Sprague-Dawley大鼠 30只，分为假手术组(n=6)、模型组(n=6)、乙酰谷酰胺组(n=6)、红花注射液组(n=6)和谷红注射液组(n=6)。采用大鼠大脑中动脉梗阻(MCAO) 2 h再灌注模型，再灌注24 h后腹腔注射给药，连续14 d。治疗后，ELISA法检测各组大鼠皮层区 VEGF 表达。结果 与假手术组相比，模型组 VEGF 表达显著降低(P<0.001)。与模型组相比，乙酰谷酰胺组和谷红注射液组VEGF的表达提高(P<0.05)。结论 谷红注射液能提高大鼠脑缺血再灌注后皮层区VEGF的表达，可能是其发挥抗脑缺血再灌注损伤的作用机制之一。

关键词: 脑缺血再灌注, 谷红注射液, 血管内皮生长因子, 大鼠

Abstract: Objective To study the effects of Guhong injection on the expression of vascular endothelial growth factor (VEGF) in the cortex 14 days after cerebral ischemia-reperfusion. Methods 30 male Sprague-Dawley rats were divided into sham group (n=6), ischemia group (n=6), aceglutamide injection group (n=6), Honghua injection group (n=6) and Guhong injection group (n=6). The middle cerebral arteries of all the rats were occluded for 2 hours and reperfusion, except the sham group. Drugs were administered once a day 24 hours after reperfusion. The expression of VEGF in cortex was detected with enzyme-linked immunosorbent assay (ELISA) 14 days after reperfusion. Results The expression of VEGF decreased in the ischemia group compared with the sham group (P<0.001), and it increased both in the aceglutamide and Guhong injection groups compared with the ischemia group (P<0.05). Conclusion Guhong injection can significantly increase the expression of VEGF in the cortex 14 days after ischemia-reperfusion, which may be one of the ways for neuro-protection.

Key words: cerebral ischemia-reperfusion, Guhong injection, vascular endothelial growth factor, rats