《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (05): 497-500.

• 特稿 •    下一篇

微管相关蛋白1轻链3Ⅱ与微管相关蛋白2 在血管性痴呆大鼠海马CA1 区的表达

刘金霞，马原源，刘斌，邓春颖，李世英

  

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-05-25 发布日期:2015-05-25

Expression of Microtubule-associated Protein 1 Light Chain 3 II and Microtubule-associated Protein 2 in CA1 Area of Hippocampus of Vascular Dementia Rats

LIU Jin-xia, MA Yuan-yuan, LIU Bin, DENG Chun-ying, LI Shi-ying   

  1. First Department of Neurology, the Affiliated Hospital of Hebei United University, Tangshan, Hebei 063000, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-05-25 Online:2015-05-25

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被引次数

8

摘要： 目的观察微管相关蛋白1 轻链3Ⅱ (LC3Ⅱ)与微管相关蛋白2 (MAP-2)在血管性痴呆大鼠海马CA1 区的表达及变化情况。方法48 只Sprague-Dawley 大鼠随机分为假手术组和模型组(血管性痴呆模型)，每组大鼠又随机分为模型制备成功后1、2、4和8 周4 个亚组，每个亚组6 只。采用四血管阻断法制备大鼠血管性痴呆模型。免疫组化法检测LC3Ⅱ与MAP-2 的表达。结果与假手术组比较，模型组各时间点LC3Ⅱ表达均显著增高(P<0.001)，MAP-2 表达均显著减少(P<0.001)。模型组各时间点大鼠海马CA1 区LC3Ⅱ与MAP-2 的蛋白表达呈负相关(r=-0.723, P<0.001)。结论LC3Ⅱ蛋白在海马CA1 区过度表达，MAP-2 蛋白表达减少，这可能是血管性痴呆发生、发展的重要机制之一。

关键词: 血管性痴呆, 微管相关蛋白1轻链3, 微管相关蛋白2, 海马, 自噬, 大鼠

Abstract: Objective To observe the expression of microtubule-associated protein 1 light chain 3 II (LC3II) and microtubule-associated protein 2 (MAP-2) in CA1 area of hippocampus in vascular dementia rats. Methods 48 Sprague-Dawley rats were randomly divided into sham group and model group. Meanwhile, each group was further divided into 1, 2, 4 and 8 weeks subgroups (n=6). Vascular dementia model was established by blocking four vessels. The expressions of LC3II and MAP-2 protein were detected with immunohistochemistry in the CA1 area of hippocampus. Results The expression of LC3II significantly increased, and the expression of MAP-2 decreased in the model group compared with the sham group at every time point (P<0.001). The expression of LC3II was negatively correlated with MAP-2 at every time point in the model group (r=-0.723, P<0.05). Conclusion It may play an important role for the occurrence and development of vascular dementia that the expression of LC3II increased and MAP-2 descreased in CA1 area of hippocampus in rats.

Key words: vascular dementia, microtubule-associated protein 1 light chain 3, microtubule-associated protein 2, hippocampus, autophagy