《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (04): 426-431.

• 基础研究 • 上一篇    下一篇

有氧运动对慢性心力衰竭大鼠骨骼肌糖原和运动耐力的影响

甄洁 1，朱荣 2
   

  1. 1.郑州大学体育系，河南郑州市 450001；2.温州医科大学，浙江温州市 325035。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-25 发布日期:2015-04-25

Effects of Aerobic Exercise on Skeletal Muscle Glycogen and Exercise Endurance in Rats with Chronic Heart Failure

ZHEN Jie1, ZHU Rong2
   

  1. 1. the Physical Education Department of Zhengzhou University, Zhengzhou, Henan 450001, China; 2. Wenzhou Medical University, Wenzhou, Zhejiang 325035, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-25 Online:2015-04-25

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被引次数

13

摘要： 目的 观察 8 周有氧运动对慢性心力衰竭大鼠骨骼肌糖原含量和运动耐力的影响和可能机制。方法 30 只Sprague-Dawley大鼠分为心力衰竭安静组(H组，n=10)、心力衰竭运动组(HT组，n=10)和假手术组(S组，n=10)，采用冠状动脉结扎术复制心力衰竭模型。HE组进行 8周跑台训练。干预后用蒽酮法测定骨骼肌糖原含量，递增负荷力竭运动试验测定力竭时间，放射性同位素法测定葡萄糖摄取率、骨骼肌糖原合酶(GS)和糖原磷酸化酶(GP)活性，Western Blotting 法测定总 GS、磷酸化 GS(p-GS)、总GP以及磷酸化GP(p-GP)蛋白表达；对糖原含量和力竭时间进行相关分析。结果 与S组大鼠比较，H组骨骼肌糖原含量、力竭时间、胰岛素介导的葡萄糖摄取率、GS活性和p-GP蛋白表达量降低(P<0.05)，血清胰岛素、GP活性和p-GS蛋白表达量升高(P<0.05)；与H组比较，HT组骨骼肌糖原含量、力竭时间、胰岛素介导的葡萄糖摄取率、GS活性和p-GP蛋白表达量增加(P<0.05)，血清胰岛素、GP活性和 p-GS蛋白表达量降低(P<0.05)。糖原含量和力竭时间显著正相关(P<0.05)。结论 长期有氧运动能提高慢性心力衰竭大鼠骨骼肌糖原含量和运动耐力，可能与胰岛素敏感性改善，葡萄糖摄取增加，糖原合成增多、分解减少有关。

关键词: 心力衰竭, 有氧运动, 骨骼肌, 糖原, 运动耐力, 大鼠

Abstract: Objective To observe the effects of 8-week aerobic exercise on skeletal muscle glycogen content and exercise endurance and investigate the possible mechanism in rats with chronic heart failure. Methods 30 Sprague-Dawley rats were submitted to heart failure sedentary (H) group, heart failure traning (HT) group and sham operation (S) group. The heart failure model was established with coronary artery ligation. HT group performed an 8-week treadmill running. The skeletal muscle glycogen content was determined with anthracenone, exercise endurance with exhaust duration of graded exhausted exercise test, glucose uptake rate, activity of glycogen synthase (GS) and glycogen phosphorylase (GP) with radicisotope, protein expression of total GS, phospho-GS (p-GS), total GP and phospho-GP (p-GP) with Western Blotting. Correlation analysis was conducted between glycogen content and exhaust duration. Results The skeletal muscle glycogen, exhaust duration, glucose uptake rate, GS activity and p-GP protein reduced (P<0.05), while serum insulin, GP activity and p-GS protein raised (P<0.05) in the H group compared with those in the S group. The skeletal muscle glycogen, exhaust duration, glucose uptake rate, GS activity and p-GP protein increased (P<0.05), while serum insulin, GP activity and p-GS protein decreased (P<0.05) in the HT group compared with those in the H group. There was positive correlation between glycogen content and exhaust duration (P<0.05). Conclusion Prolonged aerobic exercise enhanced skeletal muscle glycogen content and exercise endurance in rats with chronic heart failure, which may be related to the improvement of insulin sensitivity, muscle glucose uptake, increase of glycogen synthesis and decrease of glycogen breakdown.

Key words: heart failure, aerobic exercise, skeletal muscle, glycogen, exercise endurance, rat