《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (04): 373-377.

• 专题 •    下一篇

成年大鼠重度脊髓损伤后损伤中心不同白质束的病理变化

裴晓姣 1，杨朝阳 1，郝鹏 1，饶家声 2，赵璨 2，李晓光 1
   

  1. 1.首都医科大学神经生物学系，北京市 100069；2.北京航空航天大学生物医学工程学院，北京市 100191。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-25 发布日期:2015-04-25

Pathology of White Matter Tracts at Core of Severe Spinal Cord Injury in Adult Rats

PEI Xiao-jiao1, YANG Zhao-yang1, HAO Peng1, RAO Jia-sheng2, ZHAO Can2, LI Xiao-guang1
   

  1. 1. Department of Neurobiology, Capital Medical University, Beijing 100069, China; 2. Department of Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-25 Online:2015-04-25

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被引次数

4

摘要： 目的 研究大鼠重度脊髓损伤后损伤中心不同白质束的病理变化。方法 21只成年雌性Wistar大鼠，分为正常对照组(n=3)、假手术组(n=3)和脊髓损伤组(n=15)。损伤组使用NYU脊髓损伤打击器10 g×50 mm制备大鼠T7-8节段重度脊髓损伤模型。损伤后 1 d、3 d、7 d、14 d和 28 d通过快蓝染色(LFB染色)、NF200免疫组织化学染色检测损伤脊髓的病理改变，旷场 Basso-Beattie-Bresnahan(BBB)法评价大鼠行为学变化。结果 大鼠后肢运动功能在损伤后3 d开始自发性恢复，在14~28 d达到平台期。LFB染色显示，损伤中心腹侧束白质和外侧束白质有残留，残留白质面积百分比在损伤后3 d降至最低，7~28 d为平台期。免疫组化染色显示，损伤中心不同白质束中NF200阳性轴突数目在伤后明显减少。结论 脊髓损伤后，腹侧白质残留对大鼠后肢运动功能恢复起关键作用，伤后3 d为脊髓损伤急性期保护残留白质的关键时间窗。

关键词: 脊髓损伤, 功能恢复, 残留白质, 轴突变性

Abstract: Objective To explore pathological characteristics of different white matter tracts at core of the spinal cord injury (SCI) in adult rats. Methods 21 adult female Wistar rats were divided into normal group (n=3), sham group (n=3) and lesion group (n=15). The rats of the lesion group were severely injured at T7-8 using the NYU impactor of 10 g×50 mm. The pathology of spinal cord injury was detected using Luxol fast blue (LFB) staining and NF200 immunohistochemistry staining 1 day, 3 days, 7 days, 14 days and 28 days after injury, while the hindlimbs behavior of rats was rated with Basso-Beattie-Bresnahan (BBB) scores of open-field. Results The BBB scores recovered 3 days after injury and reached a platform from 14 to 28 days after injury. LFB showed that there were spared white matters on ventral white matter (VWM) and lateral white matter (LWM). The percentage of spared white matters area decreased to the lowest 3 days after injury, and reached a platform from 7 to 28 days after injury. The number of NF200-IR axons reduced significantly in white matter tracts after SCI. Conclusion Ventral spared white matters plays a key role in the recovery of motor function in rat with SCI, and the first 3 days was a time window to protect the white matters from injury.

Key words: spinal cord injury, function recovery, spared white matter, axon degeneration