《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (03): 269-271.

• 基础研究 • 上一篇    下一篇

麻醉方法对痉挛型瘫痪大鼠动物模型构建的影响

王玉昌，庞伟，许洪伟，冯欢欢，范艳萍   

  1. 佳木斯大学康复医学院，黑龙江省小儿脑性瘫痪防治疗育中心，黑龙江佳木斯市154002。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-03-25 发布日期:2015-03-25

Anesthesia Selection for Modeling Spastic Paralysis in Rats

WANG Yu-chang, PANG Wei, XU Hong-wei, FENG Huan-huan, FAN Yan-ping   

  1. Rehabilitation Medical Research Institute of Jiamusi University, Jiamusi, Heilongjiang, 154002 , China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-03-25 Online:2015-03-25

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摘要： 目的探讨双侧颈总动脉间断性结扎术建立大鼠痉挛型瘫痪模型时的最适麻醉剂量及方式。方法Wistar 大鼠60 只，随机分成6 组，每组10 只，均行双侧颈总动脉间断性结扎术。A组予10%水合氯醛5 ml/kg 皮下注射，B组予10%水合氯醛4 ml/kg皮下注射，C组予10%水合氯醛4 ml/kg 腹腔注射，D组予10%水合氯醛3 ml/kg 皮下注射，E组予10%水合氯醛3 ml/kg 腹腔注射，F组予10%水合氯醛2 ml/kg 皮下注射。比较每组麻醉起效时间、维持时间、术中死亡率。结果A组在麻醉中全部死亡，F组无深度麻醉。B组起效时间(6.5±0.7) min，维持时间(121.4±3.9) min，死亡率0；C组起效时间(5.5±1.1) min，维持时间(122.0±3.6) min，死亡率30%；D组起效时间(9.6±0.8) min，维持时间(106.7±3.7) min，死亡率0；E 组起效时间(7.4±1.2) min，维持时间(105.3±3.5)min，死亡率20%。皮下注射总体死亡率0，腹腔注射总体死亡率25%。结论双侧颈总动脉间断性结扎术建立大鼠痉挛型瘫痪模型时，采用10%水合氯醛4 ml/kg皮下注射麻醉，大鼠麻醉死亡率低，起效时间短，维持时间长。

关键词: 痉挛型瘫痪, 水合氯醛, 麻醉, 双侧颈总动脉间断性结扎术, 大鼠

Abstract: Objective To explore the optimal dose and ways of anesthesia for creating a rat model of spastic paralysis with intermittent bilateral common carotid artery ligation. Methods 60 Wistar rats were randomly divided into groups A, B, C, D, E and F. Group A was anaesthetized with 10% chloral hydrate 5 ml/kg injected subcutaneously, while group B with 4 ml/kg subcutaneously, group C with 4 ml/kg intraperitoneally, group D with 3 ml/kg subcutaneously, group E with 3 ml/kg intraperitoneally, group F with 2 ml/kg subcutaneously. The onset and duration of anesthesia, and intraoperative and postoperative mortality were compared. Results All the rats in the group A died during anesthesia, while the group F did not achieve the depth of anesthesia, and abandoned. The onset time was (6.5±0.7) min, maintained (121.4± 3.9) min, mortality was 0 in the group B, and it was (5.5±1.1) min, (122.0±3.6) min, 30% in the group C; (9.6±0.8) min, (106.7±3.7) min, 0 in the group D, (7.4±1.2) min, (105.3±3.5) min, 20% in the group E. The overall mortality rate was 0 in the groups accepted subcutaneous injected and 25% of intraperitoneal injection. Conclusion Anesthesia with 10% chloral hydrate 4 ml/kg subcutaneous injection is optimal of lower mortality, faster onset and longer maintaining in rats for spastic paralysis model with intermittent bilateral common carotid artery ligation.

Key words: spastic paralysis, chloral hydrate, anesthesia, intermittent bilateral common carotid artery ligation, rats