《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (01): 49-053.

• 基础研究 • 上一篇    下一篇

热预处理对超负荷训练大鼠心肌蛋白激酶C表达的影响

杨翼1，李章华2，石武谛1，柳华1，张杰1
   

  1. 1.武汉体育学院健康科学学院，湖北武汉市430079；2.武汉市第三医院，湖北武汉市430060。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-01-25 发布日期:2015-01-25

Effects of Heat Preconditioning on Expression of Protein Kinase C in Myocardium of Rats after Overload Exercise

YANG Yi, LI Zhang-hua, SHI Wu-di, LIU Hua, ZHANG Jie.
  

  1. College of Health Science, Wuhan Institute of Physical Education, Wuhan, Hubei 430079,China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-01-25 Online:2015-01-25

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摘要： 目的观察热预处理(HP)对超负荷训练大鼠心肌蛋白激酶C (PKC) δ、PKCε表达的影响。方法3 月龄雄性Sprague-Dawley 大鼠25 只分为对照组(n=5)、训练组(n=10)和HP 组(n=10)。超负荷训练8 周后应用免疫组化法、Real-time PCR 和Western blotting 检测心肌组织PKCδ、PKCε表达。结果三种检测方法均显示，训练组大鼠心肌PKCδ表达较对照组和HP组增高(P<0.05)。Real-time PCR与免疫组化检测结果显示，训练组PKCε表达低于对照组和HP组(P<0.05)；Western blotting 结果显示三组PKCε表达无显著性差异(P>0.05)。结论在热预处理心肌保护效应中，PKC可能发挥了重要的作用。

关键词: 热预处理, 心肌, 蛋白激酶C, 过度运动, 大鼠

Abstract: Objective To investigate the effects of heat preconditioning (HP) on the expression of protein kinase C (PKC) δ and PKCε in the myocardium of overload exercise rats. Methods 25 male three-month-old Sprague-Dauley rats were divided into control group (n=5), exercise group (n=10) and HP group (n=10). The expression of PKCδ and PKCε in the myocardium was detected with immunohistochemistry, real-time PCR and Western blotting 8 weeks after overload exercise. Results The expression of PKCδ significantly increased in the exercise group compared with the control group and the HP group (P<0.05). In another hand, PCR and immunohistochemistry showed the expression of PKCε in the exercise group decreased compared with the control group and the HP group (P<0.05), but it was not significantly different among the groups using Western blotting (P>0.05). Conclusion PKCs may play an important role in the HP-induced cardio-protection during overload exercise.

Key words: heat preconditioning, myocardium, protein kinase C, overload exercise, rats