《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (01): 26-030.

• 专题 • 上一篇    下一篇

不同强度运动对脑缺血再灌注大鼠学习能力及氧自由基代谢的影响

孙竹梅1，赵雅宁1，李建民1，陈长香1，赵旭1，陈乃玲2
   

  1. 1.河北联合大学康复医学院，河北唐山市063000；2.河北联合大学口腔医学院，河北唐山市063000。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-01-25 发布日期:2015-01-25

Effect of Intensity of Exercise on Learning Ability and Oxygen Free Radical Metabolism in Rats after Cerebral Ischemia-reperfusion

SUN Zhu-mei, ZHAO Ya-ning, LI Jian-min, CHEN Chang-xiang, ZHAO Xu, CHEN Nai-ling.
  

  1. Rehabilitation and Nursing College, Hebei United University, Tangshan, Hebei 063000, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-01-25 Online:2015-01-25

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摘要： 目的探讨不同强度运动对全脑缺血再灌注大鼠学习能力及海马区氧自由基代谢的影响。方法60 只雄性Sprague-Dawley 大鼠随机分成假手术组(n=15)、全脑缺血再灌注组(n=15)、有氧运动预处理组(n=15)、力竭运动预处理组(n=15)。分别在术后1 d、3 d、7 d，HE染色观察海马区神经细胞形态变化，穿梭箱评测大鼠学习能力，羟胺法测定大鼠脑组织海马区超氧化物歧化酶的活性，硫代巴比妥酸法测定丙二醛水平。结果与假手术组比较，其他各组存活的神经元数目、主动回避反应率、超氧化物歧化酶活性显著降低(P<0.001)，被动回避潜伏期、丙二醛水平显著升高(P<0.001)。其中，全脑缺血再灌注组存活的神经元数目、主动回避反应率及超氧化物歧化酶的活性显著低于有氧运动预处理组(P<0.001)，显著高于力竭运动预处理组(P<0.001)，被动回避潜伏期及丙二醛水平显著高于有氧运动预处理组(P<0.001)，低于力竭运动预处理组(P<0.001)。结论规律的有氧运动有利于保护脑缺血大鼠的学习能力，而力竭运动则产生负面影响，可能与运动对氧自由基代谢的调节有关。

关键词: 脑缺血再灌注, 学习, 有氧运动, 力竭运动, 氧自由基, 大鼠

Abstract: Objective To explore the effetc of different intensity of exercise on learning ablility and oxygen free radical metabolism in rats after cerebral ischemia-reperfusion (I/R). Methods 60 male Sprague-Dawley rats were randomly divided into sham group, I/R group, aerobic exercise preconditioning group and exhaustive exercise preconditioning group. The morphological changes of neural cells in hippocampus were observed with HE staining, the learning ablility was assessed with shuttle box, the activity of superoxide dismutase and malondialdehyde level in hippocampus were measured with hydroxylamine method and TBA method respectively 1, 3, 7 days after injury. Results The number of survival neurons, active avoidance reaction and activity of superoxide dismutase decreased, and the latency of passive avoidance and malondialdehyde levels increased in all the other groups compare with the sham group (P<0.001). Further more, the number of surviving neurons, active avoidance reaction rate and the activity of superoxide dismutase were less in the I/R group than in the aerobic exercise preconditioning group (P<0.001), and more than in the exhaustive exercise preconditioning group (P<0.001), while the latency of passive avoidance and the level of malondialdehyde was more than in the aerobic exercise preconditioning group (P<0.001), and less than in the exhaustive exercise preconditioning group (P<0.001). Conclusion Regular aerobic exercise is beneficial to protect the learning ability from cerebral I/R in rats, but exhaustive exercise may be negative, which may associated with the metabolism of oxygen free radical in hippocampus impacted by exercise.

Key words: cerebral ischemia-reperfusion, learning, aerobic exercise, exhaustive exercise, oxygen free radical, rats