《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (01): 22-025.

• 专题 • 上一篇    下一篇

运动预处理对脑缺血再灌注大鼠血清炎症因子水平的影响

朱路文1，叶涛2，吴孝军2，姜云飞2，唐强1
   

  1. 1.黑龙江中医药大学附属第二医院，黑龙江哈尔滨市150001；2.黑龙江中医药大学，黑龙江哈尔滨市150040。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-01-25 发布日期:2015-01-25

Effects of Exercise Preconditioning on Inflammatory Response in Serum in Rat after Cerebral Ischemia-reperfusion

ZHU Lu-wen,YE Tao, WU Xiao-jun, JIANG Yun-fei, TANG Qiang.
   

  1. The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin,Heilongjiang 150001, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-01-25 Online:2015-01-25

PDF (PC)

摘要： 目的探讨运动预处理对脑缺血再灌注大鼠血清白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6)及肿瘤坏死因子α(TNF-α)含量的影响。方法雄性Sprague-Dawley 大鼠24 只分为运动预处理组(n=8)、模型组(n=8)、假手术组(n=8)。线栓法制备大鼠大脑中动脉阻塞(MCAO)缺血再灌注模型。再灌注后2 h、24 h 分别行神经功能缺损评分。随后取材，HE染色观察大鼠缺血侧脑组织病理形态变化，酶联免疫吸附法检测血清TNF-α、IL-1β及IL-6 的含量。结果脑缺血再灌注后24 h，运动预处理组神经功能评分较模型组改善(P<0.05)，血清TNF-α、IL-1β及IL-6 含量明显降低(P<0.01)；脑缺血区皮质病理损伤减轻，间质水肿程度减轻，细胞排列较整齐，缺血区变性和坏死的神经元数量明显减少。结论运动预处理可以降低急性脑缺血再灌注大鼠炎症反应，降低神经功能缺损。

关键词: 脑缺血再灌注, 运动预处理, 肿瘤坏死因子α, 白细胞介素-1β, 白细胞介素-6, 炎症反应

Abstract: Objective To investigate the effect of exercise preconditioning on serum level of tumor necrosis factor α (TNF-α), interleukin (IL) -1β and IL-6 in rats after cerebral ischemia-reperfusion (I/R). Methods 24 male Sprague-Dawley rats were randomly divided into exercise preconditioning group (n=8), model group (n=8) and sham group (n=8). The middle cerebral arteries were occluded for 120 min and re-perfused. All the rats were evaluated with neurological deficit score 2 hours, 24 hours after I/R. The serum levels of TNF-α, IL-1β and IL-6 was detected with enzyme-linked immunosorbent, and the pathology was observed with HE staining 24 hours after I/R. Results The neurological deficit score decreased in the exercise preconditioning group compared with that in the model group, as well as the serum TNF-α, IL-1β and IL-6 (P<0.05) 24 hours after I/R. The pathological damage and interstitial edema alleviated in cerebral ischemia cortical, and the degeneration and necrosis of the neurons in the ischemic area significantly reduced in the exercise preconditioning group. Conclusion Exercise preconditioning may inhibit inflammatory response in I/R rats to protect neurological function from impairment.

Key words: cerebral ischemia-reperfusion, exercise preconditioning, tumor necrosis factor α, interleukin-1β, interleukin-6, inflammatoryresponse