《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (01): 1-004.

• 专题 •    下一篇

莫诺苷对大鼠脑缺血再灌注皮层Wnt信号通路转录因子表达的影响

艾厚喜1，孙芳玲1，侯虹丽1,2，张丽1，王文1
   

  1. 1.首都医科大学宣武医院动物实验室，北京市老年病医疗研究中心，北京脑重大疾病研究院，北京市100053；2.河北北方学院，河北张家口市075000。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-01-25 发布日期:2015-01-25

Effects of Morroniside on Wnt Signaling-related Transcription Factors in Ischemic Ipsilateral Cortex of Rats after Cerebral Ischemiareperfusion

AI Hou-xi, SUN Fang-ling, HOU Hong-li, ZHANG Li, WANG Wen. Xuanwu
  

  1. Hospital of Capital Medical University,Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-01-25 Online:2015-01-25

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被引次数

17

摘要： 目的研究莫诺苷对脑缺血再灌注7 d 患侧皮层Wnt信号通路相关转录因子神经发生素2 (Ngn2)、Pax6、Tbr2 表达的影响。方法健康成年雄性Sprague-Dawley 大鼠随机分为假手术组，模型组，莫诺苷小、中、大剂量组，每组3 只。线栓法制备大鼠大脑中动脉阻塞模型，造模后3 h 予莫诺苷30 mg/kg、90 mg/kg、270 mg/kg每天1 次灌胃。Western blotting 法分析术后7 d 皮层Ngn2、Pax6、Tbr2 的表达。结果与假手术组相比，模型组皮层Ngn2 表达升高(P<0.05)；与模型组相比，莫诺苷中、高剂量组Ngn2 表达明显升高(P<0.01)。模型组皮层Pax6 的表达与假手术组无显著性差异，莫诺苷中、高剂量组Pax6 表达升高(P<0.05)。各组间Tbr2 的表达无显著性差异。结论莫诺苷能增加脑缺血再灌注后皮层Ngn2、Pax6的表达，提示有促进神经发生的作用。

关键词: 莫诺苷, 脑缺血再灌注, 转录因子, Wnt信号通路, 神经发生素2, Pax6, Tbr2, 大鼠

Abstract: Objective To study the effects of morroniside on the expression of Wnt signaling-related transcription factors neurogenin 2 (Ngn2), Pax6 and Tbr2 in the ischemic ipsilateral cortex 7 days after cerebral ischemia-reperfusion in rats. Methods 15 male Sprague-Dawley rats were randomly divided into sham group (n=3), ischemia group (n=3), and morroniside groups (low, medium and high dosage groups, n=3). The middle cerebral artery were occluded for 30 min, and re-perfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg 3 hours after operation. The expression of Ngn2, Pax6 and Tbr2 in the ischemic ipsilateral cortex were detected with Western blotting analysis 7 days after operation. Results The expression of Ngn2 increased in the ischemia group compared with the sham group (P<0.05), and it further increased the morroniside groups of medium and high dosage compared with the ischemia group (P<0.01). There was no significant difference between the ischemia group and sham group in the expression of Pax6, while it increased the morroniside groups of medium and high dosage compared with the ischemia group (P<0.01). There was no significant difference among all the groups in the expression of Tbr2. Conclusion Morroniside could increase the expression of Ngn2 and Pax6 in the ischemic ipsilateral cortex 7 days after ischemia-reperfusion in rats, suggesting promoting the neurogenesis after ischemia.

Key words: morroniside, cerebral ischemia-reperfusion, transcription factors, Wnt signaling, neurogenin 2, Pax 6, Tbr2, rats