《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (3): 233-235.

• 论文 • 上一篇    下一篇

红景天对大鼠脑缺血再灌注c-Fos表达及神经细胞凋亡的影响

陈江君，刘宁，陈军   

  1. 兰州大学第一医院神经内科，甘肃兰州市730000。
  • 收稿日期:2013-07-15 修回日期:2013-09-11 出版日期:2014-03-25 发布日期:2014-03-25

Effects of Rhodiola on Expression of c-Fos and Neuronal Apoptosis after Cerebral Ischemia/Reperfusion Injury in Rats

CHEN Jiang-jun, LIU Ning, CHEN Jun.   

  1. Department of Neurology, First Hospital of Lanzhou University, Lanzhou 730000, Gansu, China
  • Received:2013-07-15 Revised:2013-09-11 Published:2014-03-25 Online:2014-03-25

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摘要： 目的探讨红景天对大鼠脑缺血再灌注后脑组织中c-Fos 表达水平及神经细胞凋亡的影响。方法Wistar 大鼠120 只，分为假手术组、模型组和干预组(红景天)，各组再分为3 h、6 h、12 h、24 h、48 h 共5 个亚组，每个亚组8 只大鼠。后两组采用大脑中动脉线栓法制备局灶性脑缺血再灌注模型，干预组术前红景天每天0.672 g/kg 灌胃15 d。参考Longa 法在大鼠麻醉清醒后进行评分，应用免疫组化、TUNEL法检测各组c-Fos 表达及神经细胞凋亡。结果模型组和干预组缺血脑组织中c-Fos 表达明显增加(P<0.01)，于再灌注48 h 达高峰；与模型组相比，干预组c-Fos 表达减少(P<0.05)，神经元凋亡明显减少(P<0.01)，神经功能缺损评分下降(P<0.05)。结论红景天能抑制大鼠脑缺血再灌注后脑组织中c-Fos 表达，减少神经元凋亡，减轻缺血再灌注损伤。

关键词: 红景天, 缺血再灌注, c-Fos, 神经元, 凋亡

Abstract: Objective To investigate the effect of Rhodiola Rosae P. E on the expression of c-Fos and the apoptosis of neuronal in rat brain after ischemia/reperfusion (I/R) injury. Methods 120 Wistar rats were divided into sham group, model group and intervention group,subgrouped as 3 h, 6 h, 12 h, 24 h, and 48 h. The model and intervention groups underwent middle cerebral artery occlusion, and the latter accepted Rhodiola 0.672 g/kg daily for 15 d. They were assessed with Longa's score. The expression of c-Fos and apoptosis of neuronal were measured with immunohistochemistry and TUNEL. Results The expression of c-Fos in the ischemic area increased after cerebral ischemia/reperfusion (P<0.01), peaking at 24 h after injury, and decreased in the intervention group (P<0.01), with the decrease of neuron apoptosis and Longa's score (P<0.01). Conclusion Rhodiola protects the brain tissue against ischemia/reperfusion injury by inhibiting the expression of c-Fos and apoptosis in rats.

Key words: Rhodiola, ischemia/reperfusion, c-Fos, neuron, apoptosis