《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (10): 916-921.

• 论文 • 上一篇    下一篇

碱性成纤维细胞生长因子-壳聚糖载体诱导成年大鼠骨髓间充质干细胞向神经细胞的转化

涂晓萌，段红梅，饶家声，杨朝阳，李晓光   

  1. 1.北京航空航天大学生物与医学工程学院，北京市100191；2.首都医科大学神经生物学系，北京市100069。
  • 收稿日期:2012-10-22 修回日期:2013-08-22 出版日期:2013-10-25 发布日期:2013-10-25
  • 通讯作者: 李晓光

Bone Marrow Mesenchymal Stem Cells' Transformation into Nerve Cells Induced by Basic Fibroblast Growth Factor-Chitosan Carrier

TU Xiao-meng, DUAN Hong-mei, RAO Jia-sheng, et al.   

  1. School of Biological and Medical Engineering, Beijing Institute of Technology,Beijing 100191, China
  • Received:2012-10-22 Revised:2013-08-22 Published:2013-10-25 Online:2013-10-25

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被引次数

2

摘要： 目的探索碱性成纤维细胞生长因子(bFGF)-壳聚糖载体对成年大鼠骨髓间充质干细胞(rMSCs)向神经细胞转化的诱导作用。方法采用免疫组织化学进行定性观察，并定量计数rMSCs 诱导转化为神经干细胞、神经元和星形胶质细胞等神经细胞，噻唑蓝(MTT)比色法定量检测诱导后细胞的活性。结果rMSCs经bFGF-壳聚糖载体诱导后，表达神经干细胞标记物nestin、神经元标记物β-tubulin Ⅲ和星形胶质细胞标记物胶质纤维酸性蛋白(GFAP)，nestin 在bFGF-壳聚糖组中高表达，9 d 时达到最大值49.40%。结论bFGF-壳聚糖载体可以诱导成年rMSCs高比例地分化为神经干细胞。

关键词: 骨髓间充质干细胞, 碱性成纤维细胞生长因子, 壳聚糖, 神经转化, 神经干细胞, 神经元, 星形胶质细胞, 大鼠

Abstract: Objective To explore the induction of basic fibroblast growth factor (bFGF)-chitosan carrier transforming the adult rat bone marrow mesenchymal stem cells (rMSCs) into nerve cells. Methods rMSCs were detected qualitatively and counted quantitatively by immunohistochemistry after they were induced into nerve cells, such as neural stem cells neurons and astrocytes. The methyl thiazolyl tetrazolium (MTT) chromometry assay was carried out to determine the cell viability. Results rMSCs induced by bFGF-chitosan carrier expressed neural stem cell marker nestin, neuron marker β-tubulin Ⅲ and astrocytes marker glial fibrillary acidic protein (GFAP). Nestin expressed more in the bFGF-chitosan group, and reached its maximum (49.40%) at the 9th day. Conclusion bFGF-chitosan carrier can induce the adult rMSCs differentiate into neural stem cells in a high proportion.

Key words: bone marrow mesenchymal stem cell, basic fibroblast growth factor, chitosan, nerve transformation, neural stem cells, neurons, astrocytes, rats