《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (09): 820-823.

• 论文 • 上一篇    下一篇

单核细胞趋化蛋白-1 在内源性神经干细胞迁移中的作用

丁鹏，周长胜，戴孝森，陆斌，王崇谦，王伟民，王进昆   

  1. 昆明医科大学第一附属医院神经外科，云南昆明市650032。
  • 收稿日期:2013-01-30 修回日期:2013-03-09 出版日期:2013-09-25 发布日期:2013-09-25
  • 通讯作者: 王进昆

Effects of Monocyte Chemoattractant Protein-1 on Migration of Endogenous Neural Stem Cells

DING Peng, ZHOU Chang-sheng,DAI Xiao-sen, et al.   

  1. Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China
  • Received:2013-01-30 Revised:2013-03-09 Published:2013-09-25 Online:2013-09-25

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摘要： 目的探讨单核细胞趋化蛋白-1(MCP-1)在神经干细胞体内迁移中的作用。方法雄性Sprague-Dawley 大鼠制备脑皮质微量注射脂多糖脑损伤大鼠模型，于造模后3 d、5 d、7 d 灌注取脑，采用免疫组织化学方法检测MCP-1 和nestin 表达。结果造模后，MCP-1 表达增高，5 d 时达到高峰；7 d 时，可在损伤区发现nestin 阳性细胞。结论MCP-1 可能趋化内源性神经干细胞向脑损伤区迁移。

关键词: 脑损伤, 神经干细胞, 单核细胞趋化蛋白-1, 迁移, 大鼠

Abstract: Objective To investigate the effects of monocyte chemoattractant protein-1 (MCP-1) on the migration of neural stem cells in vivo. Methods The brain injured rat model was established with cortical micro-injection of lipopolysaccharide in Sprague-Dawley rats. The tissue of brain was immunohistochemistrically stained to detect the expression of MCP-1 and nestin 3 d, 5 d, and 7 d after modeling. Results The expression of MCP-1 increased, and reached a peak 5 d after modeling. The nestin-positive cells was found in the lipopolysaccharide injected area 7 d after modeling. Conclusion MCP-1 may induce the endogenous neural stem cells migrate to lipopolysaccharide injection area in vivo.

Key words: cerebral injury, neural stem cells, monocyte chemoattractant protein-1, migration, rats