《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (1): 27-29.

• 论文 • 上一篇    下一篇

督脉电针对脊髓损伤大鼠生长相关蛋白43 表达的影响

李兵奎，曾彬，常巍，王达义，杨琪，王晓勋，余连军，刘涛   

  1. 1.湖北医药学院附属太和医院，a.脊柱外科；b.生命科学研究所；c.病理科，湖北十堰市442000；2.湖北医药学院附属东风公司总医院内科，湖北十堰市442000。
  • 收稿日期:2013-04-10 修回日期:2013-05-02 出版日期:2014-01-25 发布日期:2014-01-25
  • 通讯作者: 常巍

Effects of Du Meridian Electroacupuncture on Growth Associated Protein- 43 Expression in Rats after Spinal Cord Injury

LI Bing-kui, ZENG Bin, CHANG Wei, et al.   

  1. Department of Spinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei,China
  • Received:2013-04-10 Revised:2013-05-02 Published:2014-01-25 Online:2014-01-25

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摘要： 目的研究督脉电针对大鼠脊髓损伤后生长相关蛋白43(GAP-43)基因及蛋白表达的影响。方法利用多中心急性脊髓损伤打击器建立Sprague-Dawley 大鼠T11脊髓损伤模型，造模成功后分为对照组(A 组，n=18)和督脉电针组(B 组，n=18)。造模后1 周，B 组接受督脉电针治疗每天1 次。两组大鼠于造模后1、2、4、8 周行BBB 后肢运动功能评分；造模后2、4、8 周，采用RT-PCR检测GAP-43 mRNA表达，采用免疫组化染色检测GAP-43 蛋白表达。结果造模后，与A组比较，B组BBB评分明显升高(P<0.01)，GAP-43 mRNA相对表达量明显升高(P<0.01)，GAP-43 蛋白阳性表达明显增强(P<0.01)。结论督脉电针可促进脊髓损伤大鼠GAP-43 表达。

关键词: 脊髓损伤, 督脉电针, 生长相关蛋白43

Abstract: Objective To study the effect of Du meridian electroacupuncture on growth associated protein-43 (GAP-43) expression in rats after spinal cord injury (SCI). Methods The SCI models were established with MASCIS Impactor at T11 segment in Sprague-Dawley rats. They were equally divided into control group (group A, n=18) and Du meridian electroacupuncture group (group B, n=18). Group B received electroacupuncture once a day since 1 week after SCI. They were evaluated with the Basso-Beattie-Bresnahan (BBB) score 1, 2, 4, 8 weeks after SCI. The mRNA and protein of GAP-43 was detected with RT-PCR and immunohistochemistry 2, 4, 8 weeks after SCI. Results Compared with group A, the BBB score, the expression of GAP-43 mRNA and protein increased in group B after SCI (P<0.01). Conclusion Du meridian electroacupuncture can promote the expression of GAP-43 after spinal cord injury.

Key words: spinal cord injury, Du meridian electroacupuncture, growth associated protein-43