《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (08): 729-733.

• 论文 • 上一篇    下一篇

丁基苯酞对弥漫性脑创伤大鼠运动功能及学习记忆能力的影响

李建民，赵雅宁，薛承景，陈长香，李淑杏   

  1. 河北联合大学康复医学院，河北唐山市063000。
  • 收稿日期:2012-08-23 修回日期:2013-06-12 出版日期:2013-08-25 发布日期:2013-08-25
  • 通讯作者: 赵雅宁

Effects of 3-n-Butylphthalide on Neuromotor Function and Learning-Memory Function after Severe Diffuse Brain Injury in Rats

LI Jian-min, ZHAO Ya-ning, XUE Cheng-jing, et al.   

  1. Department of Rehabilitation College, Hebei United University, Tangshan 063000, Hebei,China
  • Received:2012-08-23 Revised:2013-06-12 Published:2013-08-25 Online:2013-08-25

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摘要： 目的研究丁基苯酞对弥漫性脑创伤大鼠运动功能及学习记忆能力的影响。方法128 只雄性Sprague-Dawley 大鼠随机分为假手术组、创伤组、丁基苯酞低剂量组和丁基苯酞高剂量组，每组各32 只。假手术组不致伤，丁基苯酞低、高剂量组分别注射丁基苯酞80 mg/kg 和160 mg/kg，每24 小时注射1 次。采用Marmarou 法建立大鼠弥漫性颅脑损伤模型。术后24 h、48 h、72 h 取大脑皮层冠状缝左右0.2 cm组织，光镜和电镜下观察脑组织超微结构变化，对大鼠综合运动能力以及学习记忆功能进行评测。结果与创伤组比较，丁基苯酞低、高剂量组大鼠脑组织超微结构，包括神经元内细胞器、轴索及毛细血管等超微结构的损伤程度减轻，计数存活神经元密度明显增加(P<0.01)；大鼠逃避潜伏期时间明显缩短(P<0.01)，72 h 亚组穿越平台次数明显增多(P<0.01)；综合运动能力评分提高(P<0.05)。上述指标变化在高剂量组更为显著。结论丁基苯酞可改善弥漫性脑创伤后运动和学习记忆能力损伤，其机制与减少神经元丢失，减轻脑组织超微结构损害有关。

关键词: 弥漫性脑创伤, 学习, 运动, 超微结构, 大鼠

Abstract: Objective To study the effects of 3-n-butylphthalide (NBP) on motor function and learning and memory ability in rats with diffuse brain injury (DBI). Methods 128 male Sprague-Dawley rats were randomly divided into sham operation group, traumatic group,low-dose NBP treatment group and high-dose NBP treatment group with 32 rats in each group. Every group was divided into 24 h, 48 h and 72 h subgroups. DBI rat model was established according to the description of Marmarou's diffused brain injury. The histopathologic changes of cerebral tissue (0.2 cm bilateralis coronal line) were observed by light and electron microscope at 24 h, 48 h and 72 h after injury. Morris water maze and rolling test were performed daily at 24, 48 and 72 h (time points). Results Compared with the model group, the damage of brain tissue decreased, and the survival nerve cells increased (P<0.01). The behavioral tests showed that the latency to find the platform shortened (P<0.01) and the frequency of crossing the platform increased in the 72 h subgroup (P<0.01). The general movement ability enhanced in NBP groups (P<0.05). All the indexes were more significant in high-dose NBP treatment group. Conclusion NBP can improve neurological function and learning and memory function after brain injury and the molecular mechanisms is related to the decrease of the nerve cells lose and traumatic cerebral ultrastructure damage.

Key words: diffuse brain injury, learning, motor, ultrastructure, rats