《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (05): 448-451.

• 论文 • 上一篇    下一篇

6 周高强度跑台运动对大鼠关节液及滑膜白细胞介素-1β的影响

赵景新，王瑜，何曼，金宇，白伦浩   

  1. 1.承德医学院附属医院，a.骨外四科；b.康复科，河北承德市067000；2.中国医科大学附属盛京医院关节运动骨科，辽宁沈阳市110001。
  • 收稿日期:2012-09-17 修回日期:2012-10-17 出版日期:2013-05-25 发布日期:2013-05-25
  • 通讯作者: 白伦浩

Expression of Interleukin-1β in Synovia and Synovium of Rats after High Intensity Exercise

ZHAO Jing-xin, WANG Yu, HE Man, et al.   

  1. Department of Orthopedics, Affiliated Hospital of Chengde Medical College, Chengde 067000, Heibei, China
  • Received:2012-09-17 Revised:2012-10-17 Published:2013-05-25 Online:2013-05-25

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摘要： 目的探讨高强度跑台运动对大鼠关节液及滑膜白细胞介素-1β(IL-1β)的影响。方法将24 只雄性Sprague-Dawley 大鼠随机分为安静对照组和高强度运动组各12 只。高强度运动组进行6 周高强度跑台运动。取各组大鼠膝关节股骨内侧髁软骨及滑膜组织，HE 染色观察其组织变化，免疫组化法检测IL-1β在大鼠滑膜中的表达，酶联免疫吸附法(ELISA)检测大鼠关节液中IL-1β水平。结果高强度运动组大鼠膝关节出现关节软骨损伤和滑膜炎表现，关节液中IL-1β的表达高于安静对照组(P<0.05)，滑膜组织中IL-1β的表达明显高于安静对照组(P<0.01)。结论高强度运动导致的关节软骨损伤、滑膜炎症，以及关节液、滑膜中IL-1β的异常表达可能是创伤性关节炎发生的机制之一。

关键词: 运动, 白细胞介素-1β, 骨关节炎, 滑膜, 滑液

Abstract: Objective To study the expression of interleukin-1β (IL-1β) in synovia and synovium of rats after high intensity exercise.Methods 24 healthy male Sprague-Dawley rats were randomly divided into control group and high intensity group, and the latter were forced to run 60 min at the speed of 28 m/min for 6 weeks. The histological changes of cartilage from the lesion areas of medial femoral condyle and synovium of knee joint were examined with HE stain and the expression of IL-1β in synovium with immunohistochemistry, in synovial fluid with the enzyme-linked immunosorbent assay (ELISA). Results The articular cartilage of knee were injured and synovitis were found in high intensity group. The expression of IL-1β both in synovial fluid and in synovium of the knee were more in high intensity group than in control group (P<0.05). Conclusion High intensity exercise could cause aticular cartilage injury, synovitis and over expression of IL-1β in synovia and synovium, which may associated with post-traumatic osteoarthritis.

Key words: exercise, interleukin-1β, osteoarthritis, synovium, synovia