《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (05): 444-447.

• 论文 • 上一篇    下一篇

不同穴组电针对局灶性脑梗死大鼠神经行为学及Neurocan 表达的影响

钟艺华，唐显军，李光勤，黄浩然   

  1. 1.重庆市肿瘤研究所神经内科，重庆市400031；2.重庆医科大学附属第一医院神经内科，重庆市400016。
  • 收稿日期:2012-12-20 修回日期:2012-12-26 出版日期:2013-05-25 发布日期:2013-05-25
  • 通讯作者: 李光勤

Effects of Electroacupuncture at Different Acupoints on Neuroethology and Expression of Neurocan after Focal Cerebral Infarction in Rats

ZHONG Yi-hua, TANG Xian-jun, LI Guang-qin, et al.   

  1. Department of Neurology, Chongqing Tumor Institute, Chongqing 400031,China
  • Received:2012-12-20 Revised:2012-12-26 Published:2013-05-25 Online:2013-05-25

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摘要： 目的比较电针不同经络穴组对局灶性脑梗死大鼠神经行为学及neurocan 表达的干预效果。方法将健康成年雄性Sprague-Dawley 大鼠85 只随机分为5 组：假手术组(n=5)、模型组(n=20)、水沟-百会组(n=20)、肝俞-肾俞组(n=20)和足三里-曲池组(n=20)；模型组和各电针组线栓法制作大鼠大脑中动脉闭塞模型，随机再分为脑缺血1 d、3 d、7 d、14 d 和21 d 共5 个亚组。神经行为学采用Longa 评分，RT-PCR及免疫组化测定缺血侧皮质neurocan mRNA和蛋白表达。结果假手术组无神经功能障碍；各电针组大鼠在缺血3 d 后神经行为学评分逐渐恢复，7~21 d 时较模型组明显改善(P<0.05)。模型组neurocan mRNA和蛋白表达在缺血1 d 后逐渐增强，14 d 达到峰值，21 d 时有所下降，但仍维持高水平表达(P<0.01)；各电针组在缺血3 d 后neurocan mRNA和蛋白表达均较模型组低(P<0.05)，其中水沟-百会和肝俞-肾俞组在缺血14 d 时neurocan mRNA和蛋白表达较足三里-曲池组低(P<0.05)。结论局灶性脑梗死大鼠缺血侧皮质neurocan mRNA和蛋白表达明显上调。急性期给予电针刺激能下调其表达水平，改善神经行为学评分；其中电针水沟-百会穴和肝俞-肾俞穴较足三里-曲池穴治疗效果更好。

关键词: 脑梗死, 电针, neurocan, 轴突再生, 神经行为学

Abstract: Objective To compare the effects of electroacupuncture (EA) at different acupoints on the neuroethology and expression of neurocan after focal cerebral infarction in rats. Methods The healthy male adult Sprague-Dawley rats were randomly divided into sham group (n=5), model group (n=20), Shuigou-Baihui (SB) group (n=20), Ganshu-Shenshu (GS) group (n=20) and Zusanli-Quchi (ZQ) group.All the rats were modeled with middle cerebral artery occlusion (MCAO) except sham group, and were randomly divided into 5 sub-groups as 1 d, 3 d, 7 d, 14 d and 21 d after cerebral ischemia. Their neuroethology was evaluated with Longa's score, and the expressions of neurocan mRNA and protein were detected with RT-PCR and immunohistochemistry in ischemic cortex. Results No dysfunction was found in the sham group. The neurological function recovered gradually 3 d after modeled, and improved significantly 7~21 d after modeled in each EA group compared with those in the model group (P<0.05). The expression of neurocan mRNA and protein gradually increased 1 d after modeled in the model group, peaked 14 d after modeled, and remained high level 21 d after modeled (P<0.01). The expressions of neurocan mRNA and protein were less in each EA group than in the model group 3 d after modeled (P<0.05), and were less in the SB group and GS group than in ZQ group (P<0.05). Conclusion The expression of neurocan increased significantly in ischemic cortex. EA may down-regulate the expression of neurocan after acute cerebral infarction, and improve the neuroethology. EA at SB and GS is more effective than at ZQ.

Key words: cerebral infarction, electroacupuncture, neurocan, axon regeneration, neuroethology