《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (9): 824-826.

• 论文 • 上一篇    下一篇

体外冲击波对兔膝软骨细胞增殖的影响

赵喆1,2，刘春梅1，白晓东1，邢更彦1   

  1. 1.武警总医院骨科，北京市100039；2.武警山西总队朔州支队，山西朔州市036002。
  • 收稿日期:2012-03-29 修回日期:2012-06-05 出版日期:2012-09-25 发布日期:2012-09-25
  • 通讯作者: 邢更彦

Effect of Extracorporeal Shock Wave on Chondrocyte Proliferation of Rabbit Knee in Vitro

ZHAO Zhe, LIU Chun-mei, BAI Xiao-dong, et al   

  1. Department of Orthopaedic Surgery, General Hospital of Chinese People's Army Police Forces, Beijing 100039, China
  • Received:2012-03-29 Revised:2012-06-05 Published:2012-09-25 Online:2012-09-25

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摘要： 目的体外观察体外冲击波(ESW)对软骨细胞增殖的影响。方法酶法消化获得正常兔膝软骨细胞，体外分别施加0、1×105、1.5×105、2×105、2.5×105、3×105、3.5×105 Pa ESW 0、300、600、900 次。倒置显微镜观察，HE 染色观察，CCK-8 染色筛选ESW促进软骨细胞增殖最佳压强和次数。结果1.5×105 Pa、600 次ESW干预后，软骨细胞增殖活性达最高；随压强、次数升高，软骨细胞增殖活性明显下降。结论软骨细胞是对ESW应力敏感细胞。ESW在一定压强和次数下显著促进软骨细胞增殖。

关键词: 体外冲击波, 软骨细胞, 细胞增殖, 应力细胞

Abstract: Objective To observe the effect of extracorporeal shock wave (ESW) on proliferation of chondrocyte in vitro. Methods Chondrocyte from normal rabbit knee using enzymatic digestion was treated with ESW of 0, 1×105, 1.5×105, 2×105, 2.5×105, 3×105, 3.5×105 Pa 0, 300, 600, 900 times in vitro. The pressure and frequency of ESW most promoting chondrocyte proliferation were determined with cartilage cell morphology, HE staining and CCK-8 assay. Results Chondrocyte proliferated most under 1.5×105 Pa and 600 times of ESW, and decreased as the pressure and the frequency increased. Conclusion Chondrocyte is stress-sensitive in vitro. ESW can promote chondrocyte proliferation in certain pressure and frequency

Key words: extracorporeal shock wave, chondrocyte, proliferation, stress cells