《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (7): 615-618.

• 论文 • 上一篇    下一篇

人脐带源神经干细胞的培养和分化

周剑云1,2，孙炜1,2，张新1,2，王建军1,2，陈慧1,3，张庆俊4   

  1. 1.首都医科大学康复医学院，北京市100068；2.中国康复研究中心北京博爱医院神经外科，北京市100068；3.中国康复研究中心医学所，北京市100068；4.北京大学人民医院神经外科，北京市100044。
  • 收稿日期:2011-11-02 修回日期:2012-05-18 出版日期:2012-07-25 发布日期:2012-07-25

Conversion of Human Umbilical Cord Mesenchymal Stem Cells to Neural Stem Cell-like Cells in Vitro

ZHOU Jian-yun, SUN Wei,
ZHANG Xin, et al.   

  1. Department of Neurosurgery, Capital Medical University School of Rehabilitation Medicine, Beijing Bo'ai Hospital, China Rehabilitation Research Centre, Beijing 100068, China
  • Received:2011-11-02 Revised:2012-05-18 Published:2012-07-25 Online:2012-07-25

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被引次数

7

摘要： 目的研究诱导人脐带间充质干细胞(hucMSC)分化为神经干细胞样细胞(hucNSC)的方法。方法体外培养hucMSC，流式细胞仪鉴定细胞表型的表达。用碱性成纤维细胞生长因子、表皮生长因子添加B27 诱导，流式细胞学、免疫细胞化学鉴定。用胶质源性神经营养因子(GDNF)、白介素-1β (IL-1β)和全反式维甲酸(ATRA)等诱导分化，免疫组化染色、RT-PCR 鉴定。结果hucMSC 表达CD105、CD44、CD29。诱导后聚集成细胞球悬浮生长，并不断增殖，流式细胞仪鉴定失去hucMSC 的表面标志物特征，而表达Nestin、CD133。再经RA、GDNF、IL-1β诱导，细胞表达胶质纤维酸性蛋白、神经元特异性烯醇化酶和酪氨酸羟化酶。结论hucMSC能分化为具有神经干细胞样生长、增殖、分化特征的细胞。

关键词: 人脐带间充质干细胞, 神经干细胞, 多巴胺能神经元, 分化

Abstract: Objective To study the efficient conversion of human umbilical cord mesenchymal stem cells (hucMSC) into a neural stem cell-like population (hucNSC). Methods MSCs derived from umbilical cord were cultured and identified with fluorescence-activated cell sorting (FACS). They were induced with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and B27, and identified with FACS and immunocytochemistry. Then they were induced with glial-derived neurotrophic fator(GDNF), interleukin-1β (IL-1β) and all-trans retinoic acid (ATRA), and identified with immunocytochemistry and RT-PCR. Results hucMSC expressed CD105, CD44 and CD29. Induced
with EGF, bFGF and B27, they expressed CD133 and Nestin. Induced with GDNF, IL-1β and ATRA, they expressed glial fibrillary acidic protein, neuron specific enolase and tyrosine hydroxylase. Conclusion hucMSC may be induced into hucNSC, namely the cells with the characteristic growth, proliferation and differentiation like NSCs.

Key words: human umbilical cord mesenchymal stem cells, neural stem cell, dopaminergic neurons, differentiation