《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (5): 420-422.

• 论文 • 上一篇    下一篇

减重步行训练结合电针对脊髓损伤大鼠损伤组织脑源性神经营养因子的影响

刘帅1，邢艳丽2， 孟巍1   

  1. 1.黑龙江中医药大学，黑龙江哈尔滨市 150040；2.黑龙江中医药大学附属第二医院，黑龙江哈尔滨市 150040。
  • 收稿日期:2011-11-08 修回日期:2011-12-16 出版日期:2012-05-25 发布日期:2012-05-25

Effect of Body Weight Support Treadmill Training and Electroacupuncture on Expression of Brain-derived Neurotrophic Factor inSpinal Cord Injuried Rats

LIU Shuai, XING Yan-li, MENG Wei.   

  1. Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang,China
  • Received:2011-11-08 Revised:2011-12-16 Published:2012-05-25 Online:2012-05-25

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被引次数

18

摘要： 目的探讨减重步行训练结合电针对大鼠脊髓损伤脑源性神经营养因子(BDNF)表达的影响。方法健康成年SD大鼠56 只建立T11脊髓横断损伤模型，分为对照组、电针组、针康组各16 只，其余8 只为空白组。造模后3 d 开始治疗。分别在治疗后1 d、7 d、14 d、21 d 对各组大鼠进行BBB评分，用免疫组化法测定脊髓组织中BDNF 的表达。结果治疗后，针康组BBB评分高于电针组及对照组(P<0.05)；BDNF 水平高于电针组和对照组(P<0.05)。结论电针结合康复训练可以促进脊髓损伤的恢复。脊髓损伤的恢复与脊髓组织中BDNF 的表达有关。

关键词: 脊髓损伤, 电针, 减重步行训练, 脑源性神经营养因子, 大鼠

Abstract: Objective To investigate the mechanism of body weight support treadmill training (BWSTT) and electroacupuncture (EA)for spinal cord injury. Methods 56 adult SD rats were divided into control group (n=16), EA group (n=16), BWSTT-EA group (n=16) andblank group (n=8). They were treated 3 d after operation, and assessed with Basso-Beattie-Bresnahan (BBB) scale and the brain-derived neurotrophicfactor (BDNF) was measured in injured tissue with real time immunohistochemisty assay 1 d, 7 d, 14 d, 21 d after treatment. ResultsThe score of BBB was significantly higher in the BWSTT- EA group than in control group and EA group 1 d, 7 d, 14 d, 21 d after treatment(P<0.05), as well as the expression of BDNF (P<0.05). Conclusion BWSTT and EA can promote the recovery of spinal cord injury,which may associate with the expression of BDNF in injured tissue.

Key words: spinal cord injury, electroacupuncture, body weight support treadmill training, brain-derived neurotrophic factor, rats