《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (5): 401-405.

• 论文 •    下一篇

血管内栓塞大脑中动脉制作非人灵长类卒中慢性期模型的研究

王强1,2a，张通1,2a，赵春禹1,2a，徐建民1,2b，温枚1,2c，裴志胜1,2c   

  1. 1.首都医科大学康复医学院，北京市100068；2.中国康复研究中心北京博爱医院，a.神经康复中心；b.影像科；c.基础所，北京市 100068。
  • 收稿日期:2011-08-01 修回日期:2011-11-10 出版日期:2012-05-25 发布日期:2012-05-25

Nonhuman Primate Chronic Stroke Model with Middle Cerebral Artery Endovascular Embolism

WANG Qiang, ZHANG Tong,ZHAO Chun-yu, et al.   

  1. Neurological Rehabilitation Centre, Capital Medical University School of Rehabilitation Medicine, Beijing Bo'ai Hospital,China Rehabilitation Research Centre, Beijing 100068, China
  • Received:2011-08-01 Revised:2011-11-10 Published:2012-05-25 Online:2012-05-25

PDF (PC)

被引次数

11

摘要： 目的建立非人灵长类脑梗死慢性期动物模型。方法雄性成年恒河猴10 只，血管内介入栓塞动物利手对侧大脑中动脉。依栓塞节段不同，分为M1 段栓塞组(n=3)、上干栓塞组(n=5)和下干栓塞组(n=2)。通过标准神经量表和小槽食丸取回任务评定运动功能。结果M1 段栓塞组均在急性期死亡。上干栓塞组均存活，MRI 可见梗死灶，急性期一侧肢体瘫痪，其中4 只功能障碍在慢性期持续存在，另外1 只运动功能恢复。下干栓塞组急性期一侧肢体瘫痪，运动缺陷恢复迅速且完全。结论大脑中动脉上干栓塞动物模型存活率高，慢性期存在运动功能障碍，适合于慢性期研究。

关键词: 脑梗死, 慢性期, 非人灵长类, 动物模型, 血管内栓塞

Abstract: Objective To establish chronic cerebral infarction animal model in nonhuman primate. Methods 10 adult male rhesus monkeyswere embolized the middle cerebral artery (MCA) in contra-lateral of handedness, and divided into M1 segment embolism group (n=3), upper trunk embolism group (n=5), and lower trunk embolism group (n=2). Acute neurological deficit was evaluated with standard neurologicalscale, and the motor function in chronic stage was assessed with a task of retrieving food pill in wells. Results Animals in M1 segmentembolism group all died 38~62 h after surgery. Upper trunk embolism group survived, and MRI showed front parietal cortex infarctioncontra-lateral paralyzed side. All of them paralyzed one side in acute stage, and 4 of them persisted dysfunction in chronic stage, that couldnot finish the task of retrieving food pill in wells; only one completed the task. The lower trunk embolism group paralyzed one side in acutestage, but recovered quickly and completely, that finished the task within 7 d. Conclusion Embolism of MCA upper trunk can cause infarctionof precise and proper size with one side limb dysfunction in the acute stage and long-term dysfunction in most animals, which is feasiblefor treatment and neural plasticity research in recovery.

Key words: cerebral infarction, chronic stage, nonhuman primate, animal model, endovascular embolism