《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (2): 131-133.

• 论文 • 上一篇    下一篇

莫诺苷对二磷酸腺苷诱导兔血小板聚集钙离子的影响

艾厚喜1，左玮1,2，王晓锋2,3，张丽2，季晖2，陈乃宏3，王文1   

  1. 1.首都医科大学宣武医院，北京市 100053；2.中国药科大学，江苏南京市 210009；3.中国医学科学院药物所，北京市 100050。
  • 收稿日期:2011-08-22 修回日期:2011-11-14 出版日期:2012-02-25 发布日期:2012-02-25
  • 通讯作者: 王文

Morroniside Inhibiting Ca2+ after Platelet Aggregation Induced by Adenosine Diphosphate in Rabbits

AI Hou-xi, ZUO Wei, WANG Xiao-feng, et al.   

  1. Xuanwu Hospital of Capital Medical University, Beijing 100053, China
  • Received:2011-08-22 Revised:2011-11-14 Published:2012-02-25 Online:2012-02-25

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摘要： 目的观察莫诺苷对二磷酸腺苷(ADP)诱导兔血小板聚集后Ca2++浓度的影响。方法利用钙离子荧光探针(Fura-2 AM)法，通过记录5 min 之内Fura-2 在激发波长340 nm和380 nm处的荧光强度比值，检测不同条件下ADP 诱导血小板聚集后Ca2++的变化。结果与空白对照组相比，莫诺苷能显著抑制由ADP 诱导兔血小板聚集后Ca2++的升高(P<0.001)。结论莫诺苷可能通过降低Ca2++的上升达到抗ADP 诱导的兔血小板的聚集，从而改善体外血液流变学。

关键词: 莫诺苷, 血小板聚集, 钙, 兔

Abstract: Objective To explore the effects of morroniside on Ca2++ in the condition of platelet aggregation in rabbits induced by adenosine diphosphate (ADP). Methods The mobilization of cytosolic-free calcium after platelet aggregation induced by ADP was detected by Ca2++-sensitive fluorescent indicator, Fura-2 AM and time scan measurement. Results Compared with the controls, morroniside significantly inhibited the increase of Ca2++ induced by ADP (P<0.001). Conclusion Morroniside acts as an effective platelet aggregative antagonist by inhibiting the increase of platelet Ca2++.

Key words: morroniside, platelet aggregation, calcium, rabbits