《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (2): 101-103.

• 论文 •    下一篇

针康法对局灶性海马缺血大鼠学习记忆能力及突触素表达的影响

赵振峰，金翊思   

  1. 哈尔滨医科大学附属第五医院康复医学科，黑龙江大庆市 163318。
  • 收稿日期:2011-08-04 修回日期:2011-10-08 出版日期:2012-02-25 发布日期:2012-02-25

Effects of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Learning and Memory Ability and Expression of Synaptophysin in Rats with Hippocampal Infarction

ZHAO Zhen-feng, JIN Yi-si.   

  1. The fifth Affiliated Hospital of Harbin Medical University,Harbin 163318, Heilongjiang, China
  • Received:2011-08-04 Revised:2011-10-08 Published:2012-02-25 Online:2012-02-25

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摘要： 目的探讨头穴丛刺结合康复训练(针康法)对海马梗死大鼠学习记忆能力及突触素表达的影响。方法将50 只大鼠随机分为假手术组、模型组、头穴丛刺组、康复组、针康组，每组10 只。采用光化学法诱导局灶性海马缺血大鼠模型，术后24 h 进行干预，治疗14 d 后采用Y-型迷宫评价大鼠学习记忆能力，免疫组化法观察突触素表达情况。结果与假手术组比较，各组大鼠学习能力下降，突触素表达增加(P<0.05)。与模型组比较，头穴丛刺组、康复组、针康组学习能力明显提高(P<0.01)，突触素表达增加(P<0.05)，且针康组优于头穴丛刺组和康复组(P<0.05)。结论针康法能促进学习记忆能力的恢复，上调突触素的表达，其疗效优于单纯头穴丛刺和单纯康复训练。

关键词: 脑缺血, 学习, 记忆, 突触素, 大鼠

Abstract: Objective To explore the effects of cluster needling of scalp acupuncture combined with rehabilitation on learning and memory ability and expression of synaptophysin in rats with hippocampal infarction. Methods 50 rats were randomly divided into sham operation group, model group, acupuncture group, rehabilitation group and acupuncture with rehabilitation group, 10 rats in each group. Photochemical method was used to induce hippocampal infarction model. 24 h after modeling, they were intervened respectively. Y-type maze was used to evaluate the learning and memory ability and immunohistochemistry method was used to observe the expression of synaptophysin after 14 d intervention. Results Compared with the sham operation group, the learning and memory ability in the other groups declined,and the expression of synaptophysin increased (P<0.05). Compared with the model group, both the learning and memory ability and the expression of synaptophysin increased in the acupuncture group, the rehabilitation group and the acupuncture with rehabilitation group (P<0.05). The acupuncture with rehabilitation group was better than the acupuncture group and the rehabilitation group (P<0.05). Conclusion The cluster needling of scalp acupuncture combined with rehabilitation can promote the learning and memory ability and the expression of synaptophysin in rats.

Key words: cerebral ischemia, learning, memory, synaptophysin, rats