《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (4): 361-363.

• 论文 • 上一篇    下一篇

儿童哮喘与ORMDL3基因多态性关系的研究

孙婧，李乔俊，徐飞，王卉   

  1. 解放军总医院第一附属医院儿科，北京市 100048。
  • 收稿日期:2010-03-23 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25

Relationship between ORMDL3 Gene Polymorphism and Asthma in Children

SUN Jing, LI Qiao-jun, XU Fei, et al.   

  1. Department of Pediatrics, First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China
  • Received:2010-03-23 Revised:1900-01-01 Published:2010-04-25 Online:2010-04-25

PDF (PC)

被引次数

32

摘要： 目的探讨儿童哮喘与ORMDL3基因多态性的关系。方法选取2005年10月至2009年3月解放军总医院以及解放军总医院附属医院的哮喘病儿童178例(分轻症组和重症组)，同期健康体检或外科非炎症儿童患者129例作为对照组。抽取静脉血3 ml，采用PCR及限制性酶切方法对ORMDL3基因的rs7216389位点的基因型进行测定。结果3组基因型(CC＼TC＼TT)分布频率比较有显著性差异(P<0-05)。对于TT型，轻症组与对照组相比无显著性差异(P>0-05)，重症组的比率高于轻症组和对照组(P<0-05)。哮喘儿童T等位基因频率高于对照组。结论儿童哮喘与ORMDL3基因多态性有一定的相关性。

关键词: 儿童, 哮喘, ORMDL3, 基因多态性, rs7216389

Abstract: ObjectiveTo explore the relationship between ORMDL3 gene polymorphism and Asthma in children.Methods178 cases of children with asthma were divided into mild group and severe group, and 129 cases of children were treated as normal control group who tested blood samples in the PLA General Hospital and its Affiliated Hospital in the recent 4 years. ORMDL3 gene locus rs7216389 genotype was detected, including PCR, restriction endonuclease analysis and bio-statistical aspects of analysis.ResultsThere was significant difference (P<0-05) in 3 genotypes (CC＼TC＼TT). There was no significant difference in TT between the mild group and the control group (P>0-05). The rate of severe group was higher than that in the mild and the control groups(P<0-05). Asthmas in children showed higher T allele frequency.ConclusionORMDL3 gene polymorphism showed a certain relevance with asthma in children.

Key words: children, asthma, ORMDL3, gene polymorphism, rs7216389