《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (07): 625-627.

• 专题 • 上一篇    下一篇

外周N-甲基-D-天门冬氨酸受体在福尔马林外周注射引起长时程痛敏中的作用

程涓1;张策2   

  1. 1. 北京中医药大学东直门医院病理科，北京市 100700; 2. 山西医科大学生理学教研室，山西太原市 030001
  • 收稿日期:2009-05-19 出版日期:2009-07-01 发布日期:2009-07-01

Peripheral N-methyl-D-aspartate Receptors Involved in Long-term Hyperalgesia Induced by Peripheral Injection of Formalin in Rats

CHENG Juan,ZHANG Ce   

  1. Department of Pathology, Dongzhimen Hospital, Beijing University of Chinese Medical Science, Beijing 100700, China
  • Received:2009-05-19 Published:2009-07-01 Online:2009-07-01

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摘要： 目的 观察外周N-甲基-D-天门冬氨酸（NMDA）受体对福尔马林外周注射所致长时程痛敏的作用。方法 雄性SD大鼠脚掌注射福尔马林，之前注射或不注射MK-801。免疫组化方法检测脊髓背角小胶质细胞OX-42表达，测定热辐射痛行为。结果 注射MK-801侧脊髓背角Ⅰ、Ⅱ层小胶质细胞OX-42阳性表达水平较对侧（仅注入福尔马林侧）明显降低。单独注入福尔马林后第3天发生热痛敏，第7天达高峰，第10天仍未完全恢复至正常值; 外周应用MK-801后，热痛敏反射潜伏期延长。结论 NMDA受体参与长时程痛敏的产生和维持。

关键词: 长时程痛敏, 福尔马林实验, 小胶质细胞, N-甲基-D-天门冬氨酸（NMDA）受体, MK-801, OX-42, 大鼠

Abstract: Objective To investigate the role of peripheral N-methyl-D-aspartate (NMDA) receptors in the long-term hyperalgesia induced by peripheral injection of formalin in rats. Methods Formalin was injected in the paws of the SD rats with or without the MK-801 pre-injection. OX-42 expressions in the dorsal horn were observed, and the paw withdrawal thermal latency was measured. Results The OX-42 expression decreased and the paw withdrawal thermal latency prolonged in rats with MK-801 pre-injection. Conclusion Peripheral NMDA receptors may be involved in the long-term hyperalgesia.

Key words: long-term hyperalgesia, formalin test, microglia, N-methyl-D-aspartate (NMDA) receptors, MK-801, OX-42, rats