《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (11): 963-965.

• 基础研究 • 上一篇    下一篇

实时荧光定量逆转录聚合酶链反应检测火器伤骨折愈合过程中骨形态发生蛋白的表达

李秉胜1a; 陈英剑1b; 张强1a; 范长春1a; 郑吉波1a; 蔡锦方   

  1. 1.济南军区总医院a.骨创伤外科;b.实验诊断科,山东济南市 250031
  • 收稿日期:2006-04-06 出版日期:2006-11-01 发布日期:2006-11-01

Expression of Bone Morphogenetic Protein-2 in Gunshot Fracture Healing Detected with Real Time Fluorescence Quantitative Reverse Transcript Polymerase Chain Reaction

LI Bing-sheng, CHEN Ying-jian, ZHANG Qiang, et al   

  1. General Hospital of Jinan Military Command,Ji'nan 250031, Shandong, China
  • Received:2006-04-06 Published:2006-11-01 Online:2006-11-01

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摘要： 目的应用实时荧光定量逆转录聚合酶链反应(RT-PCR)检测兔火器伤骨折愈合过程中骨形态发生蛋白(BMP)-2mRNA水平。方法建立外固定架固定一期治疗兔股骨火器伤骨折的动物模型和BMP-2 mRNA表达水平的SYBR G reenⅠ荧光定量RT-PCR方法,分别于术后第1、2、3、4周取断端组织,通过实时荧光定量RT-PCR检测标本标本中BMP-2 mRNA水平。结果在对照组,创伤后的兔骨组织BMP-2 mRNA表达水平在1周开始增高,在第2周达到高峰后下降,第4周即可恢复正常。而实验组,受枪伤的兔骨组织BMP-2 mRNA表达水平在第3周表达最高,与对照组相比,升高较慢,表达量也偏低(P<0.05)。结论BMP-2mRNA SYBR G reenⅠ荧光定量RT-PCR可以很好地反应枪击伤对兔骨组织BMP-2 mRNA表达水平的影响。枪击伤后的兔骨组织BMP-2 mRNA表达水平与对照组相比升高较慢,表达量也偏低。

关键词: 火器伤骨折, 骨形态发生蛋白(BMP-2), 实时荧光定量, 逆转录聚合酶链反应(RT-PCR)

Abstract: ObjectiveTo detect the levels of bone morphogenetic protein-2(BMP-2) mRNA in gunshot fracture healing of the rabbits.MethodsA model of primary treating gunshot fracture of the rabbit with external fixator and the real time fluorescence quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR) technology with SYBR Green I were established.The levels of BMP-2 mRNA 1,2,3 and 4 weeks after operation were detected with FQ-RT-PCR respectively.ResultsIn the control group,BMP-2 mRNA of the bone tissue began to rise at the first week after fracture and the peak of mRNA expression appeared at the second week.The expression returned to normal at the forth week.In the experimental group,the BMP-2 mRNA rose more slowly that it arrived peak stage at the third week and was significantly lower in experimental group than that in control group at the same phases.ConclusionReal time FQ-RT-PCR is a good method for measuring the levels of BMP-2 mRNA during gunshot fracture healing.The mRNA expression rose more slowly and was significantly lower in gunshot fracture than in common fracture at the same phases.

Key words: gunshot fracture, bone morphogenetic protein-2(BMP-2), real time fluorescence quantity, reverse transcription polymerase chain reaction(RT-PCR)