《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (11): 960-962.

• 基础研究 • 上一篇    下一篇

雌激素对血清饥饿诱导成骨细胞凋亡基因表达的影响

唐孝明1; 裴福兴2; 李胜富2; 曾建成2; 张耀明1; 刘仲前1; 庞健1   

  1. 1.四川省人民医院骨科,四川成都市 610072;2.四川大学华西医院骨科,四川成都市 610041
  • 收稿日期:2006-04-13 出版日期:2006-11-01 发布日期:2006-11-01

Effect of Estrogen on Expression of Osteoblast Apoptosis Related Genes Induced with Serum Hungry

TANG Xiao-ming, PEI Fu-xing, LI Sheng-fu, et al   

  1. Department of Orthopaedics, Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan, China
  • Received:2006-04-13 Published:2006-11-01 Online:2006-11-01

PDF (PC)

被引次数

8

摘要： 目的探讨雌激素对血清饥饿诱导的体外培养大鼠成骨细胞凋亡基因bc l-2、bax、fas表达的影响,为明确雌激素抑制由血清饥饿诱导的大鼠成骨细胞凋亡的机理提供依据。方法新生SD大鼠颅骨第2、3代成骨细胞随机分为对照组、血清饥饿组、血清饥饿+雌激素组3组,每组细胞分别培养1 d、2 d、3 d、5 d、7 d、14 d后做免疫组化染色,计数各组Bc l-2、Bax、Fas阳性细胞率。结果对照组成骨细胞有少量Bc l-2、Bax、Fas表达;与对照组比较,血清饥饿组细胞Bax和Fas表达升高(P<0.05),高峰期为14 d,Bc l-2的表达无明显变化(P>0.05);与血清饥饿组比较,血清饥饿+雌激素组细胞Bax和Fas表达降低(P<0.05),高峰期仍为14d,Bc l-2的表达升高(P<0.05)。结论血清饥饿使成骨细胞Bax和Fas表达增强,而对Bc l-2的表达无明显影响;雌激素能抑制血清饥饿增强成骨细胞Bax和Fas表达的作用,并使Bc l-2的表达增加,从而抑制血清饥饿诱导的大鼠成骨细胞凋亡。

关键词: 雌激素, 成骨细胞, 细胞凋亡, 基因

Abstract: ObjectiveTo explore the mechanism of estrogen inhibiting osteoblast apoptosis induced with serum hungry.MethodsOsteoblasts of the second or third generation from newly born SD rats calvaria were divided randomly into three groups: control group,serum hungry group,serum hungry with estrogen group.Cells of each group were incubated for 1,2,3,5,7 or 14 d,and then were stained immunohistochemically.The rates of positive cells of each group were analyzed.ResultsThere was a little positive expression of Bax,Bcl2 and Fas in control group.The expression of Bax and Fas were significantly increased(P<0.05)in serum hungry group,peak time was 14 d,but the expression of Bcl-2 were not affected.Compared with that of serum hungry group,the expression of Bax and Fas significantly decreased(P<0.05) in serum hungry and estrogen group,peak time was still 14 d,while that of Bcl-2 increased(P<0.05).ConclusionSerum hungry can increase the expression of Bax and Fas in osteoblast,that can be inhibited by estrogen.Estrogen can also increase the expression of Bcl-2 in osteoblast.All of these may play a role in inhibiting osteoblast apoptosis induced with serum hungry.

Key words: estrogen, osteoblast, apoptosis, gene