《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (11): 954-956.

• 基础研究 • 上一篇    下一篇

自体施万细胞神经膜管移植修复大鼠脊髓损伤的实验研究

南丰; 李靖年; 董建俐; 王鸿飞; 东海潮; 高延明   

  1. 大连医科大学附属第二医院骨科,辽宁大连市 116027
  • 收稿日期:2006-05-29 出版日期:2006-11-01 发布日期:2006-11-01

Repairing of Spinal Cord Injury with Schwann Cell Neurilemma Channel in Rats

NAN Feng, LI Jing-nian, DONG Jian-li, et al   

  1. The Second Affiliated Hospital, Dalian Medical University, Dalian 116027, Liaoning, China
  • Received:2006-05-29 Published:2006-11-01 Online:2006-11-01

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摘要： 目的探讨自体施万细胞神经膜管(SCNC)移植对脊髓半横断损伤的修复作用。方法成鼠30只,随机分3组:10只造模后行SCNC移植(A组);10只造模但不移植(B组);10只不造模也不移植作正常对照组(C组)。下段胸髓半切法造模。术后8周每周行动物行为测试和斜板试验;术后6周测定皮层体感诱发电位(CSEP);术后8周行组织学观察及计算机图像分析。结果A组、C组可测出CSEP波形,B组未测出。行为测试、斜板试验及图像分析A组、C组与B组差异显著,A组与C组之间斜板试验有显著差异。A组通过脊髓损伤处的再生神经纤维数量明显多于B组。结论自体施万细胞神经膜管移植有助于引导再生轴突生长通过,对损伤脊髓再生有较明显的促进作用。

关键词: 施万细胞神经膜管(SCNC), 脊髓损伤, 大鼠

Abstract: ObjectiveTo apply the Schwann cell neurilemma channel(SCNC) in promoting the regeneration of injuried spinal cord.MethodsSpinal cords of rats after laminectomy at T8/9 were divided in 3 groups randomly: 10 rats were transplanted with SCNC(Group A);10 rats only made models(Group B);10 rats were as normal control group(Group C).All rats received behaviour survey and inclined plane test every week 8 weeks after operation.6 weeks after operation,they were measured with cortical somatosensory evoked potential(CSEP).And 8 weeks after operation,all specimens were studied histologically.ResultsCSEP could be recorded in group A and C,but not in group B.There were marked differenties between group A or C and group B in behavior survey,inclined plane test.Amount of nerve fibers regenerated through the injury gap in group A was significantly larger than that in group B.ConclusionSCNC could facilitate axonal regeneration and promote the repairing of injuried spinal cord.

Key words: Schwann cell neurilemma channel(SCNC), spinal cord injury, rats