《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (11): 923-925.

• 专题　脑损伤康复 • 上一篇    下一篇

还原型谷胱甘肽对大鼠局灶性脑梗死后丙二醛、谷胱甘肽过氧化物酶、超氧化物歧化酶表达的影响

鲁雅琴1; 宋焱峰2; 侯一平2   

  1. 1.兰州大学第一医院神经内科,甘肃兰州市 73000;2.兰州大学基础医学院解剖学教研室,甘肃兰州市 730000
  • 收稿日期:2006-01-09 出版日期:2006-11-01 发布日期:2006-11-01

Effect of Reduced Glutathione on Expression of Malondialdehyde,Glutathione Peroxidase and Superoxide Dismutase after Focal Cerebral Infarction in Rats

LU Ya-qin, SONG Yan-feng, HOU Yi-ping   

  1. Department of Neurology, No.1Hospital of Lanzhou University,Lanzhou 730000, Gansu, China
  • Received:2006-01-09 Published:2006-11-01 Online:2006-11-01

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摘要： 目的观察还原型谷胱甘肽对大鼠短暂性局灶性脑缺血时丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)的影响。方法采用大脑中动脉线栓法制备大鼠局灶性脑缺血模型,随机分为假手术组、缺血模型组和还原型谷胱甘肽治疗组(1200 mg/kg),缺血2 h再灌注6 h后观测各组的神经行为变化,脑梗死体积,脑组织病理学变化,脑组织MDA含量和SOD、GSH-PX活性。结果还原型谷胱甘肽可以改善大鼠局灶性脑缺血引起的神经行为障碍,减少脑梗死体积(P<0.05);可以降低脑组织MDA含量,提高脑组织GSH-PX、SOD活力(P<0.05)。结论外源性谷胱甘肽可通过提高GSH-PX、SOD活力,降低MDA含量,增强机体清除自由基的能力,及减少大鼠脑组织氧自由基的堆积而发挥对脑缺血再灌注时的保护作用。

关键词: 还原型谷胱甘肽(GSH), 丙二醛(MDA), 谷胱甘肽过氧化物酶(GSH-PX), 超氧化物歧化酶(SOD), 局灶性脑梗死, 大鼠

Abstract: ObjectiveTo observe the effect of reduced glutathione(GSH) on expression of malondialdehyde(MDA),glutathione peroxidase(GSH-PX) and superoxide dismutase(SOD) after focal cerebral infarction in rats.MethodsRat models of middle cerebral artery occlusion(MCAO) were estabilished with thread after 2-hour ischemia and 6-hour reperfusion.Rats were divided at random into three groups,i.e.,sham-operated,control and treatment group(with GSH 1200 mg/kg) respectively.After the rats model was performed,neurology deficit score,the size of brain infarct region and the change of brain tissue pathologic were evaluated.Contents of MDA and activity of SOD and GSH-PX were detected with spectrophotometer.ResultsCompared with the control group,GSH can ameliorate neurological deficit score and decrease infarct volume induced by MCAO.GSH may reduce contents of MDA and improve activity of SOD and GSH-PX in brain tissue.ConclusionGSH may reduce contents of MDA and improve activity of SOD and GSH-PX so as to enhance capability of eliminating oxygen free radical,and play a neuroprotective effect after cerebral focal ischemia reperfusion.

Key words: reduced glutathione(GSH), malondialdehyde(MDA), glutathione peroxidase(GSH-PX), superoxide dismutase(SOD), focal cerebral infarction, rats