《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (08): 647-648.

• 专题 脑损伤及其并发症 • 上一篇    下一篇

大鼠脑出血移植施万细胞的实验研究

张绍东; 历俊华; 万虹   

  1. 北京市神经外科研究所,北京市 100050
  • 收稿日期:2006-05-12 出版日期:2006-08-01 发布日期:2006-08-01

Experimental Study of Schwann Cells Transplanted into Cerebral Hemorrhage Area of Rats

ZHANG Shao-dong, Ll Jun-hua, WAN Hong   

  1. Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2006-05-12 Published:2006-08-01 Online:2006-08-01

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被引次数

3

摘要： 目的探讨施万细胞(SCs)移植于大鼠脑出血部位对神经再生的影响。方法体外培养SCs,5'-溴脱氧尿嘧啶(BrdU)标记后移植于脑出血模型大鼠的脑出血部位,不同时间处死动物,免疫组织化学双染检测BrdU/髓鞘碱性蛋白(MBP)和BrdU/生长相关蛋白43(GAP43)的表达。结果SCs移植1周后至13周,脑内可见BrdU/MBP双染阳性细胞;移植后第12周可见BrdU和GAP43阳性细胞,海马部位GAP43阳性细胞尤其多见。结论移植的SCs参与髓鞘形成和神经再生。

关键词: 施万细胞, 髓鞘碱性蛋白, 生长相关蛋白43, 大鼠

Abstract: ObjectiveTo investigate the effect of Schwann cells (SCs) transplanted into cerebral hemorrhage area on never restore in rat.MethodsSCs were expended and labeled with 5'-Bromodexyuridine (BrdU) in vitro, then transplanted into cerebral hemorrhage area of model rat. Double immunohistochemistry staining was used to detect the expression of BrdU/myelin basic protein (MBP) and BrdU/growth associated protein-43 (GAP-43) respectively.ResultsBrdU/MBP positive cells could be seen one week after transplantation and up to 13 weeks. GAP-43 positive cells appeared in 12 weeks and 13 weeks, which was more in Hippocamp.ConclusionGrafted SCs can participate in remyelination and promoter nerve restore.

Key words: Schwann cells, myelin basic protein (MBP), growth associated protein-43 (GAP-43), rat