《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (12): 1132-1133.

• 基础研究 • 上一篇    下一篇

体外培养大鼠星形胶质细胞划伤模型

师忠芳; 韩明; 徐立新; 董丽萍; 袁芳   

  1. 首都医科大学北京市神经外科研究所,北京市 100050
  • 收稿日期:2007-08-03 出版日期:2007-12-01 发布日期:2007-12-01

A Scratch-wound Model in Cultured Rat Astrocytes

SHI Zhong-fang, HAN Ming, XULi-xin, et al   

  1. Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China
  • Received:2007-08-03 Published:2007-12-01 Online:2007-12-01

PDF (PC)

被引次数

23

摘要： 目的复制体外培养大鼠星形胶质细胞划伤模型。方法利用传至第2代的体外培养的大鼠星形胶质细胞进行细胞划伤实验。分别于划伤前10min、划伤后1、3、6、12、24h观察细胞形态变化,并取培养上清液进行乳酸脱氢酶活性测定。结果细胞划伤后划痕两侧边缘整齐,随时间推移细胞突起逐渐向划痕区延伸,且划痕区出现星形胶质细胞。细胞划伤后乳酸脱氢酶漏出量短时间内迅速增加,之后各时间点持续增加(P<0.05),且均高于相应时间点的对照组(P<0.05)。结论细胞形态变化及培养上清液中乳酸脱氢酶漏出量证实体外培养大鼠星形胶质细胞划伤模型复制成功。

关键词: 星形胶质细胞, 细胞培养, 划伤模型, 乳酸脱氢酶

Abstract: Objective To reproduce a scratch-wound model in cultured rat astrocytes (AST).Methods The secondary cultured AST prepared from newborn Wistar rat cerebral cortex were scratched with plastic pipette tips. The morphologic change of AST was observed through microscope at 10 min before and 1, 3, 6, 12, and 24 h after injury, meanwhile the lactate dehydrogenase (LDH) leakages in the cultured medium were determined.Results Immediately after injury the edge of the scratch was lined with irregularly shaped cell. 6 h after injury the AST processes began extending to cell-free area, and elongated further at 12 and 24 h after injury, with presented of new generated cells in the denuded area. At different times after injury, the LDH leakages of the experiment group were higher than that before injury ( P<0.05), and were higher than that of the control group ( P<0.05).Conclusion According to observed AST morphologic changes and determined LDH leakages in culture medium, the scratch-wound model in cultured rat AST is successfully reproduced.

Key words: astrocyte, cell culture, scratch-wound model, lactate dehydrogenase